Bead Implantation and Delivery of Exogenous Growth Factors

Rena Kashimoto, Saya Furukawa, Sakiya Yamamoto, Akira Satoh

Research output: Chapter in Book/Report/Conference proceedingChapter


Genetic methods in axolotls (Ambystoma mexicanum) remain in their infancy which has hampered the study of limb regeneration. There is much room for advancement, especially with respect to spatiotemporal regulation of gene expression. Secreted growth factors play a major role in each stage of regeneration. The use of slow-release beads is one of the most effective methods to control the spatiotemporal expression of secretory gene products. The topical administration of secreted factors by slow-release beads may also prove effective for future applications in non-regenerative animals and for medical applications in humans, in which genetic methods are not available. In this chapter, we describe a methodology for using and implanting slow-release beads to deliver exogenous growth factors to salamanders.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • FGF
  • Gelatin
  • Limb regeneration
  • Regeneration
  • Sustained release

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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