TY - JOUR
T1 - BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats
AU - Kuramoto, Satoshi
AU - Yasuhara, Takao
AU - Agari, Takashi
AU - Kondo, Akihiko
AU - Jing, Meng
AU - Kikuchi, Yoichiro
AU - Shinko, Aiko
AU - Wakamori, Takaaki
AU - Kameda, Masahiro
AU - Wang, Feifei
AU - Kin, Kyohei
AU - Edahiro, Satoru
AU - Miyoshi, Yasuyuki
AU - Date, Isao
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology , JAPAN; and by a Research Grant from the Japan Epilepsy Research Foundation .
PY - 2011/1/12
Y1 - 2011/1/12
N2 - Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothesized that continuous administration of BDNF at low dose might be more effective for epilepsy model of animals because high dose of BDNF was used in many studies. BDNF-secreting cells were genetically made and encapsulated for transplantation. Rats receiving BDNF capsule showed significant amelioration of seizure stage and reduction of the number of abnormal spikes at 7 days after kainic acid administration, compared to those of control group. The number of BrdU and BrdU/doublecortin positive cells in the hippocampus of BDNF group significantly increased, compared to that of control group. NeuN positive cells in the CA1 and CA3 of BDNF group were significantly preserved, compared to control group. In conclusion, low dose administration using encapsulated BDNF-secreting cells exerted neuroprotective effects with enhanced neurogenesis on epilepsy model of rats. These results might suggest the importance of the dose and administrative way of this neurotrophic factor to the epilepsy model of animals.
AB - Brain-derived neurotrophic factor (BDNF) is a well neurotrophic factor with neuroprotective potentials for various diseases in the central nervous system. However several previous studies demonstrated that BDNF might deteriorate symptoms for epilepsy model of animals by progression of abnormal neurogenesis. We hypothesized that continuous administration of BDNF at low dose might be more effective for epilepsy model of animals because high dose of BDNF was used in many studies. BDNF-secreting cells were genetically made and encapsulated for transplantation. Rats receiving BDNF capsule showed significant amelioration of seizure stage and reduction of the number of abnormal spikes at 7 days after kainic acid administration, compared to those of control group. The number of BrdU and BrdU/doublecortin positive cells in the hippocampus of BDNF group significantly increased, compared to that of control group. NeuN positive cells in the CA1 and CA3 of BDNF group were significantly preserved, compared to control group. In conclusion, low dose administration using encapsulated BDNF-secreting cells exerted neuroprotective effects with enhanced neurogenesis on epilepsy model of rats. These results might suggest the importance of the dose and administrative way of this neurotrophic factor to the epilepsy model of animals.
KW - Brain-derived neurotrophic factor
KW - Epilepsy
KW - Ex vivo gene therapy
KW - Kainic acid
KW - Neurogenesis
KW - Neuroprotection
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U2 - 10.1016/j.brainres.2010.10.054
DO - 10.1016/j.brainres.2010.10.054
M3 - Article
C2 - 20971090
AN - SCOPUS:78651076964
VL - 1368
SP - 281
EP - 289
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
ER -