BDNF gene can be activated by Ca2+ signals without involvement of de novo AP-1 synthesis

Kuniaki Sano, Hikaru Nanba, Akiko Tabuchi, Tomofusa Tsuchiya, Masaaki Tsuda

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Although stimulation of N-methyl-D-aspartate receptors or voltage-dependent calcium channels induces both the activation of c-fos and brain-derived neurotrophic factor (BDNF) genes, it is not certain how the activation of these genes is related. Using primary cultures of rat hippocampal neurons, we found that exposing the cells to cycloheximide allowed subsequent activation of BDNF mRNA expression, although activation of AP-1 DNA-binding activity resulting from the c-fos induction was abolished. Super-induction of BDNF gene was also caused by cycloheximide. The estimated half-life of BDNF mRNA was approximately 2.5 hrs, which was almost identical to that of c-fos mRNA. These results indicate that nascent AP-1 is not required for the activation of BDNF gene, leading to the notion that the BDNF gene can be activated by Ca2+ signals as an immediate early gene.

Original languageEnglish
Pages (from-to)788-793
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number3
DOIs
Publication statusPublished - Dec 24 1996

Fingerprint

Brain-Derived Neurotrophic Factor
Transcription Factor AP-1
Genes
Chemical activation
Cycloheximide
Messenger RNA
Immediate-Early Genes
Calcium Channels
N-Methyl-D-Aspartate Receptors
Cell culture
Transcriptional Activation
Neurons
Half-Life
Rats
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

BDNF gene can be activated by Ca2+ signals without involvement of de novo AP-1 synthesis. / Sano, Kuniaki; Nanba, Hikaru; Tabuchi, Akiko; Tsuchiya, Tomofusa; Tsuda, Masaaki.

In: Biochemical and Biophysical Research Communications, Vol. 229, No. 3, 24.12.1996, p. 788-793.

Research output: Contribution to journalArticle

@article{6ad7bed553f64fbea8da47c9e136fe20,
title = "BDNF gene can be activated by Ca2+ signals without involvement of de novo AP-1 synthesis",
abstract = "Although stimulation of N-methyl-D-aspartate receptors or voltage-dependent calcium channels induces both the activation of c-fos and brain-derived neurotrophic factor (BDNF) genes, it is not certain how the activation of these genes is related. Using primary cultures of rat hippocampal neurons, we found that exposing the cells to cycloheximide allowed subsequent activation of BDNF mRNA expression, although activation of AP-1 DNA-binding activity resulting from the c-fos induction was abolished. Super-induction of BDNF gene was also caused by cycloheximide. The estimated half-life of BDNF mRNA was approximately 2.5 hrs, which was almost identical to that of c-fos mRNA. These results indicate that nascent AP-1 is not required for the activation of BDNF gene, leading to the notion that the BDNF gene can be activated by Ca2+ signals as an immediate early gene.",
author = "Kuniaki Sano and Hikaru Nanba and Akiko Tabuchi and Tomofusa Tsuchiya and Masaaki Tsuda",
year = "1996",
month = "12",
day = "24",
doi = "10.1006/bbrc.1996.1881",
language = "English",
volume = "229",
pages = "788--793",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - BDNF gene can be activated by Ca2+ signals without involvement of de novo AP-1 synthesis

AU - Sano, Kuniaki

AU - Nanba, Hikaru

AU - Tabuchi, Akiko

AU - Tsuchiya, Tomofusa

AU - Tsuda, Masaaki

PY - 1996/12/24

Y1 - 1996/12/24

N2 - Although stimulation of N-methyl-D-aspartate receptors or voltage-dependent calcium channels induces both the activation of c-fos and brain-derived neurotrophic factor (BDNF) genes, it is not certain how the activation of these genes is related. Using primary cultures of rat hippocampal neurons, we found that exposing the cells to cycloheximide allowed subsequent activation of BDNF mRNA expression, although activation of AP-1 DNA-binding activity resulting from the c-fos induction was abolished. Super-induction of BDNF gene was also caused by cycloheximide. The estimated half-life of BDNF mRNA was approximately 2.5 hrs, which was almost identical to that of c-fos mRNA. These results indicate that nascent AP-1 is not required for the activation of BDNF gene, leading to the notion that the BDNF gene can be activated by Ca2+ signals as an immediate early gene.

AB - Although stimulation of N-methyl-D-aspartate receptors or voltage-dependent calcium channels induces both the activation of c-fos and brain-derived neurotrophic factor (BDNF) genes, it is not certain how the activation of these genes is related. Using primary cultures of rat hippocampal neurons, we found that exposing the cells to cycloheximide allowed subsequent activation of BDNF mRNA expression, although activation of AP-1 DNA-binding activity resulting from the c-fos induction was abolished. Super-induction of BDNF gene was also caused by cycloheximide. The estimated half-life of BDNF mRNA was approximately 2.5 hrs, which was almost identical to that of c-fos mRNA. These results indicate that nascent AP-1 is not required for the activation of BDNF gene, leading to the notion that the BDNF gene can be activated by Ca2+ signals as an immediate early gene.

UR - http://www.scopus.com/inward/record.url?scp=0030600548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030600548&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.1881

DO - 10.1006/bbrc.1996.1881

M3 - Article

C2 - 8954973

AN - SCOPUS:0030600548

VL - 229

SP - 788

EP - 793

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -