Bcl-xL overexpression in human hepatocellular carcinoma.

Jota Watanabe, Fumiki Kushihata, Kazuo Honda, Katsumi Mominoki, Seiji Matsuda, Nobuaki Kobayashi

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

It is known that proliferation and apoptosis are closely related phenomena in liver cancer cells. In this study, using surgical specimens from 42 patients with hepatocellular carcinoma (HCC), we investigated the expression and localization of Bcl-xL, an antiapoptosis-related protein of the Bcl-2 family. Using Western blotting, Bcl-xL expression was detected in both cancerous and non-cancerous specimens from all of the HCC patients, and elevated Bcl-xL levels were found in cancerous specimens from two thirds of the patients. In normal human liver specimens, Bcl-xL was found mainly in the cytoplasm of hepatocytes, although it was also found in the cytoplasm of bile duct cells in Glisson's capsule by immunohistochemical staining. To the best of our knowledge, this is the first report of Bcl-xL overexpression and localization in HCC specimens. Bcl-xL was found not only in the cytoplasm of HCC cells, but also in the nuclei of some HCC cells, suggesting that Bcl-xL is involved in the progression of HCC cells in vivo.

Original languageEnglish
Pages (from-to)515-519
Number of pages5
JournalInternational Journal of Oncology
Volume21
Issue number3
Publication statusPublished - Sep 2002
Externally publishedYes

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Hepatocellular Carcinoma
Cytoplasm
Liver Neoplasms
Bile Ducts
Capsules
Hepatocytes
Western Blotting
Apoptosis
Staining and Labeling
Liver
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Watanabe, J., Kushihata, F., Honda, K., Mominoki, K., Matsuda, S., & Kobayashi, N. (2002). Bcl-xL overexpression in human hepatocellular carcinoma. International Journal of Oncology, 21(3), 515-519.

Bcl-xL overexpression in human hepatocellular carcinoma. / Watanabe, Jota; Kushihata, Fumiki; Honda, Kazuo; Mominoki, Katsumi; Matsuda, Seiji; Kobayashi, Nobuaki.

In: International Journal of Oncology, Vol. 21, No. 3, 09.2002, p. 515-519.

Research output: Contribution to journalArticle

Watanabe, J, Kushihata, F, Honda, K, Mominoki, K, Matsuda, S & Kobayashi, N 2002, 'Bcl-xL overexpression in human hepatocellular carcinoma.', International Journal of Oncology, vol. 21, no. 3, pp. 515-519.
Watanabe J, Kushihata F, Honda K, Mominoki K, Matsuda S, Kobayashi N. Bcl-xL overexpression in human hepatocellular carcinoma. International Journal of Oncology. 2002 Sep;21(3):515-519.
Watanabe, Jota ; Kushihata, Fumiki ; Honda, Kazuo ; Mominoki, Katsumi ; Matsuda, Seiji ; Kobayashi, Nobuaki. / Bcl-xL overexpression in human hepatocellular carcinoma. In: International Journal of Oncology. 2002 ; Vol. 21, No. 3. pp. 515-519.
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