bcl-2 Regulation in normal resting lymphocytes and lymphoblasts

Eisaku Kondo, Tadashi Yoshino, Shintaro Nomura, Shuji Nakamura, Kiyoshi Takahashi, Norihiro Teramoto, Kazuhiko Hayashi, Tadaatsu Akagi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Expression of the bcl-2 protein and bcl-2 mRNA at the individual cell level was semiquantitatively examined in normal quiescent peripheral blood lymphocytes and pokeweed mitogen- or concanavalin A and interleukin-2-induced lymphoblasts in vitro by microscopic fluorometry using immunofluorescence and fluorescein-labeled in situ hydridization. Approximately 90% of normal quiescent T and B lymphocytes expressed bcl-2 protein at a level which was compatible with that of bcl-2 mRNA. On the contrary, most mitogen-induced lymphoblasts showed a posttranscriptional suppression of bcl-2 protein expression. However, bcl-2 protein was not downregulated by the posttranscriptional suppression in all lymphocytes activated in vitro, but approximately 15% of the lymphoblasts still expressed bcl-2 protein at a higher level than nontransformed quiescent small lymphocytes; thus bcl-2 protein expression in lymphoblasts showed a distinct bimodal pattern. Furthermore, it was supposed that lymphoblasts with no detectable bcl-2 protein might fall into apoptosis but the remainder, expressing high levels of bcl-2 protein, could escape apoptosis. Thus, the bcl-2 gene may play an important role as a regulator of apoptosis in the human immune system.

Original languageEnglish
Pages (from-to)260-265
Number of pages6
JournalJapanese Journal of Cancer Research
Volume85
Issue number3
Publication statusPublished - Mar 1994

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Lymphocytes
Proteins
Apoptosis
bcl-2 Genes
Pokeweed Mitogens
Fluorometry
Messenger RNA
Concanavalin A
Fluorescein
Mitogens
Interleukin-2
Fluorescent Antibody Technique
Immune System
B-Lymphocytes
Down-Regulation
T-Lymphocytes
In Vitro Techniques

Keywords

  • Apoptosis
  • bcl-2
  • Lymphocyte
  • Mitogen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kondo, E., Yoshino, T., Nomura, S., Nakamura, S., Takahashi, K., Teramoto, N., ... Akagi, T. (1994). bcl-2 Regulation in normal resting lymphocytes and lymphoblasts. Japanese Journal of Cancer Research, 85(3), 260-265.

bcl-2 Regulation in normal resting lymphocytes and lymphoblasts. / Kondo, Eisaku; Yoshino, Tadashi; Nomura, Shintaro; Nakamura, Shuji; Takahashi, Kiyoshi; Teramoto, Norihiro; Hayashi, Kazuhiko; Akagi, Tadaatsu.

In: Japanese Journal of Cancer Research, Vol. 85, No. 3, 03.1994, p. 260-265.

Research output: Contribution to journalArticle

Kondo, E, Yoshino, T, Nomura, S, Nakamura, S, Takahashi, K, Teramoto, N, Hayashi, K & Akagi, T 1994, 'bcl-2 Regulation in normal resting lymphocytes and lymphoblasts', Japanese Journal of Cancer Research, vol. 85, no. 3, pp. 260-265.
Kondo E, Yoshino T, Nomura S, Nakamura S, Takahashi K, Teramoto N et al. bcl-2 Regulation in normal resting lymphocytes and lymphoblasts. Japanese Journal of Cancer Research. 1994 Mar;85(3):260-265.
Kondo, Eisaku ; Yoshino, Tadashi ; Nomura, Shintaro ; Nakamura, Shuji ; Takahashi, Kiyoshi ; Teramoto, Norihiro ; Hayashi, Kazuhiko ; Akagi, Tadaatsu. / bcl-2 Regulation in normal resting lymphocytes and lymphoblasts. In: Japanese Journal of Cancer Research. 1994 ; Vol. 85, No. 3. pp. 260-265.
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