Bauhinia purpurea - A new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease

A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30)

Ashit Baran Sarker, Tadaatsu Akagi, Ho Jong Jeon, Kenji Miyake, Ichiro Murakami, Tadashi Yoshino, Kiyoshi Takahashi, Soichiro Nose

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Abstract

Thirty-three cases of Hodgkin's disease (thirteen nodular sclerosis, four diffuse, lymphocyte predominance, and sixteen mixed cellularity) were studied with Bauhinia purpurea (BPA), peanut agglutinin (PNA), anti-Leu-M1, LN2, and Ber-H2 by the avidinbiotin-peroxidase complex (ABC) method in paraffin sections. Reed-Stemberg (RS) cells and variants were stained positively with one or more of the reagents in all cases. BPA staining was positive in 32 of 33 cases (97.0%), PNA staining was positive in 23 of 33 cases (69.7%), Leu-M1 was positive in 13 of 33 cases (39.4%), LN2 was positive in 14 of 33 cases (42.4%), and Ber-H2 was positive in 24 of 33 cases (72.7%). Many RS cells were stained moderately to strongly and were readily recognized in 31 cases (96.9%) of BPA+, 10 (43.5%) of PNA+, 8 (61.5%) of Leu-M1+, 6 (42.9%) of LN2+, and 22 (91.7%) of Ber-H2+ cases; in the remaining positive cases, the RS cells were found only after careful searching. Three staining patterns were recognized: paranuclear, diffuse cytoplasmic, and membranous. These three patterns were obtained with all markers except for LN2. LN2 showed diffuse cytoplasmic staining in most of the positive cells, and a few cells showed paranuclear deposits. BPA reactivity was not affected by formalin fixation or paraffin embedding. Except for RS cells, BPA also showed dense cytoplasmic staining reaction with macrophage-histiocytes. Sixty cases of non-Hodgkin's diffuse lymphomas (30 T-and 30 B-cell origin) were also studied. Tumor cells were not stained with BPA, PNA, and Leu-M1, but stained positively with LN2 in six T-cell lymphomas and thirteen B-cell lymphomas, and with Ber-H2 in six T-cell lymphomas and one B-cell lymphoma. In conclusion, to facilitate the detection of RS cells and related variants in paraffin sections, BPA can be accepted as a useful marker due to its high-detection rate, reproducible staining pattern, and resistance to fixatives.

Original languageEnglish
Pages (from-to)19-23
Number of pages5
JournalAmerican Journal of Pathology
Volume141
Issue number1
Publication statusPublished - Jul 1992

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Bauhinia
Reed-Sternberg Cells
Peanut Agglutinin
Hodgkin Disease
Paraffin
Staining and Labeling
T-Cell Lymphoma
B-Cell Lymphoma
Non-Hodgkin's Lymphoma
Paraffin Embedding
Fixatives
Histiocytes
Sclerosis
Formaldehyde
Peroxidase
B-Lymphocytes
Macrophages
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bauhinia purpurea - A new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease : A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30). / Sarker, Ashit Baran; Akagi, Tadaatsu; Jeon, Ho Jong; Miyake, Kenji; Murakami, Ichiro; Yoshino, Tadashi; Takahashi, Kiyoshi; Nose, Soichiro.

In: American Journal of Pathology, Vol. 141, No. 1, 07.1992, p. 19-23.

Research output: Contribution to journalArticle

Sarker, Ashit Baran ; Akagi, Tadaatsu ; Jeon, Ho Jong ; Miyake, Kenji ; Murakami, Ichiro ; Yoshino, Tadashi ; Takahashi, Kiyoshi ; Nose, Soichiro. / Bauhinia purpurea - A new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease : A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30). In: American Journal of Pathology. 1992 ; Vol. 141, No. 1. pp. 19-23.
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title = "Bauhinia purpurea - A new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease: A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30)",
abstract = "Thirty-three cases of Hodgkin's disease (thirteen nodular sclerosis, four diffuse, lymphocyte predominance, and sixteen mixed cellularity) were studied with Bauhinia purpurea (BPA), peanut agglutinin (PNA), anti-Leu-M1, LN2, and Ber-H2 by the avidinbiotin-peroxidase complex (ABC) method in paraffin sections. Reed-Stemberg (RS) cells and variants were stained positively with one or more of the reagents in all cases. BPA staining was positive in 32 of 33 cases (97.0{\%}), PNA staining was positive in 23 of 33 cases (69.7{\%}), Leu-M1 was positive in 13 of 33 cases (39.4{\%}), LN2 was positive in 14 of 33 cases (42.4{\%}), and Ber-H2 was positive in 24 of 33 cases (72.7{\%}). Many RS cells were stained moderately to strongly and were readily recognized in 31 cases (96.9{\%}) of BPA+, 10 (43.5{\%}) of PNA+, 8 (61.5{\%}) of Leu-M1+, 6 (42.9{\%}) of LN2+, and 22 (91.7{\%}) of Ber-H2+ cases; in the remaining positive cases, the RS cells were found only after careful searching. Three staining patterns were recognized: paranuclear, diffuse cytoplasmic, and membranous. These three patterns were obtained with all markers except for LN2. LN2 showed diffuse cytoplasmic staining in most of the positive cells, and a few cells showed paranuclear deposits. BPA reactivity was not affected by formalin fixation or paraffin embedding. Except for RS cells, BPA also showed dense cytoplasmic staining reaction with macrophage-histiocytes. Sixty cases of non-Hodgkin's diffuse lymphomas (30 T-and 30 B-cell origin) were also studied. Tumor cells were not stained with BPA, PNA, and Leu-M1, but stained positively with LN2 in six T-cell lymphomas and thirteen B-cell lymphomas, and with Ber-H2 in six T-cell lymphomas and one B-cell lymphoma. In conclusion, to facilitate the detection of RS cells and related variants in paraffin sections, BPA can be accepted as a useful marker due to its high-detection rate, reproducible staining pattern, and resistance to fixatives.",
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T1 - Bauhinia purpurea - A new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease

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AU - Sarker, Ashit Baran

AU - Akagi, Tadaatsu

AU - Jeon, Ho Jong

AU - Miyake, Kenji

AU - Murakami, Ichiro

AU - Yoshino, Tadashi

AU - Takahashi, Kiyoshi

AU - Nose, Soichiro

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