Basic fibroblast growth factor inhibits osteogenic differentiation of stem cells from human exfoliated deciduous teeth through ERK signaling

B. Li, C. Qu, C. Chen, Y. Liu, K. Akiyama, R. Yang, F. Chen, Y. Zhao, S. Shi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Objective: Stem cells from human exfoliated deciduous teeth (SHED) are a unique postnatal stem cell population capable of regenerating mineralized tissue and treating immune disorders. However, the mechanism that controls SHED differentiation is not fully understood. Here, we showed that basic fibroblast growth factor (bFGF) treatment attenuated SHED-mediated mineralized tissue regeneration through activation of the extracellular signal-regulated kinase (ERK) 1/2 pathway. Material and Method: The level of mineralized nodule formation was assessed by alizarin red staining. Expression levels of osteogenic genes, osteocalcin and runt-related transcription factor 2, were examined by RT-PCR. Subcutaneous implantation approach was used to assess in vivo bone formation. Downstream signaling pathways of bFGF were examined by Western blotting. Result: Activation of ERK1/2 signaling by bFGF treatment inhibited WNT/β-catenin pathway, leading to osteogenic deficiency of SHED. ERK1/2 inhibitor treatment rescued bFGF-induced osteogenic differentiation deficiency. Conclusion: These data suggest that bFGF inhibits osteogenic differentiation of SHED via ERK1/2 pathway. Blockade ERK1/2 signaling by small molecular inhibitor treatment improves bone formation of SHED after bFGF treatment.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalOral Diseases
Volume18
Issue number3
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

Keywords

  • Differentiation
  • ERK
  • SHED
  • Tissue regeneration

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)

Fingerprint Dive into the research topics of 'Basic fibroblast growth factor inhibits osteogenic differentiation of stem cells from human exfoliated deciduous teeth through ERK signaling'. Together they form a unique fingerprint.

Cite this