Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation

Kentaro Uchida, Gen Inoue, Osamu Matsushita, Kyosuke Horikawa, Hiroyuki Sekiguchi, Wataru Saito, Shotaro Takano, Hisako Fujimaki, Masayuki Miyagi, Masashi Takaso

Research output: Contribution to journalArticle

Abstract

Cell-based regenerative therapy has the potential to repair bone injuries or large defects that are recalcitrant to conventional treatment methods, including drugs and surgery. Here, we developed a multilayered cell-based bone formation system using cells coated with fibronectin-gelatin (FN-G) nanofilms. The multilayered mesenchymal cells (MLMCs) were formed after two days of culture and were shown to express higher levels of BMP-2 and VEGF compared to monolayer cultures of MCs. The MLMCs were used as a graft material in combination with a fusion protein consisting of basic fibroblast growth factor (bFGF), polycystic kidney disease (PKD) domain, and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase. In femur sites grafted with the MLMCs, significantly higher levels of callus volume and bone mineral content were observed compared to the sham controls. The callus volume and bone mineral content were further increased in femur sites grafted with bFGF-PKD-CBD/MLMCs. Taken together, these results suggest that bFGF-PKD-CBD/MLMCs, which can be simply and rapidly generated in vitro, have the potential to promote bone repair when grafted into large defect sites.

Original languageEnglish
Article number4371460
JournalBioMed Research International
Volume2017
DOIs
Publication statusPublished - 2017

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation'. Together they form a unique fingerprint.

  • Cite this

    Uchida, K., Inoue, G., Matsushita, O., Horikawa, K., Sekiguchi, H., Saito, W., Takano, S., Fujimaki, H., Miyagi, M., & Takaso, M. (2017). Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation. BioMed Research International, 2017, [4371460]. https://doi.org/10.1155/2017/4371460