TY - JOUR
T1 - Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity
AU - Kimura, Satoshi
AU - Ikeuchi, Yoshiho
AU - Kitahara, Kei
AU - Sakaguchi, Yuriko
AU - Suzuki, Takeo
AU - Suzuki, Tsutomu
N1 - Funding Information:
Grants-in-aid for scientific research on priority areas from the Ministry of Education, Science, Sports and Culture of Japan (to T.S.); JSPS Fellowship for Japanese Junior Scientists (to S.K.); the New Energy and Industrial Technology Development Organization (NEDO) (grant to T.S.). Funding for the open access charge: Japan Ministry of Education, Science, Sports and Culture.
PY - 2012/5
Y1 - 2012/5
N2 - Modifications of rRNAs are clustered in functional regions of the ribosome. In Helix 74 of Escherichia coli 23S rRNA, guanosines at positions 2069 and 2445 are modified to 7-methylguanosine(m7G) and N2- methylguanosine(m2G), respectively. We searched for the gene responsible for m7G2069 formation, and identified rlmL, which encodes the methyltransferase for m2G2445, as responsible for the biogenesis of m7G2069. In vitro methylation of rRNA revealed that rlmL encodes a fused methyltransferase responsible for forming both m7G2069 and m2G2445. We renamed the gene rlmKL. The N-terminal RlmL activity for m2G2445 formation was significantly enhanced by the C-terminal RlmK. Moreover, RlmKL had an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. In fact, we observed that RlmKL was involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD.
AB - Modifications of rRNAs are clustered in functional regions of the ribosome. In Helix 74 of Escherichia coli 23S rRNA, guanosines at positions 2069 and 2445 are modified to 7-methylguanosine(m7G) and N2- methylguanosine(m2G), respectively. We searched for the gene responsible for m7G2069 formation, and identified rlmL, which encodes the methyltransferase for m2G2445, as responsible for the biogenesis of m7G2069. In vitro methylation of rRNA revealed that rlmL encodes a fused methyltransferase responsible for forming both m7G2069 and m2G2445. We renamed the gene rlmKL. The N-terminal RlmL activity for m2G2445 formation was significantly enhanced by the C-terminal RlmK. Moreover, RlmKL had an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. In fact, we observed that RlmKL was involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD.
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U2 - 10.1093/nar/gkr1287
DO - 10.1093/nar/gkr1287
M3 - Article
C2 - 22210896
AN - SCOPUS:84861385355
VL - 40
SP - 4071
EP - 4085
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 9
ER -