Basal nitric oxide production is enhanced by hydraulic pressure in cultured human periodontal ligament fibroblasts.

C. Nakago-Matsuo, Toshihiko Matsuo, T. Nakago

Research output: Contribution to journalArticle

13 Citations (Scopus)


To understand orthodontic tooth movement and determine optimal orthodontic force from a biological viewpoint, nitric oxide production in cultured human periodontal ligament fibroblasts was measured at varying levels of hydraulic pressure. The fibroblasts in a culture flask were exposed to the controlled change in hydraulic pressure, and intracellular nitric oxide levels were measured in real time by a nitric oxide-binding fluorescent dye, diaminofluorescein-2. The fibroblasts produced a significantly larger amount of nitric oxide at the pressure of 75 and 100 mmHg, compared with the pressure of 0, 25, and 50 mmHg (P

Original languageEnglish
Pages (from-to)474-478
Number of pages5
JournalAmerican Journal of Orthodontics and Dentofacial Orthopedics
Issue number4
Publication statusPublished - Apr 2000
Externally publishedYes


ASJC Scopus subject areas

  • Dentistry(all)
  • Surgery

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