Basal nitric oxide production is enhanced by hydraulic pressure in cultured human trabecular cells

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background/aims - Nitric oxide donors reduce intraocular pressure. Human trabecular cells in culture were examined for their nitric oxide production in response to hydraulic pressure. Methods - Human trabecular cells were cultured from trabeculum tissue fragments excised during trabeculectomy and exposed to hydraulic pressure change in a culture flask connected to a glass syringe. The pressure was exerted by automatic infusion of the piston of the syringe and monitored by a pressure gauge. The intracellular nitric oxide level was measured in real time with a nitric oxide binding fluorescent dye, diaminofluorescein-2. Results - Intracellular nitric oxide levels in cultured trabecular cells showed spontaneous fluctuation during 400 seconds of observation. Peak levels of intracellular nitric oxide were significantly higher at hydraulic pressure of 30, 40, and 50 mmHg, compared with 0 and 25 mmHg (p <0.0001, one way ANOVA, and p <0.05, Tukey-Kramer test). The fluctuation was completely abolished by the presence of N-methyl-L-arginine (L-NMMA), a nitric oxide synthase inhibitor. The cultured trabecular cells were shown by immunohistochemistry to express brain nitric oxide synthase (bNOS). Conclusion - Higher levels of hydraulic pressure enhanced basal production of nitric oxide in human trabecular cells. Nitric oxide would be a physiological mediator in the regulation of intraocular pressure.

Original languageEnglish
Pages (from-to)631-635
Number of pages5
JournalBritish Journal of Ophthalmology
Volume84
Issue number6
DOIs
Publication statusPublished - Jun 2000

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Nitric Oxide
Pressure
Cultured Cells
Syringes
Intraocular Pressure
Nitric Oxide Synthase
omega-N-Methylarginine
Trabeculectomy
Nitric Oxide Donors
Fluorescent Dyes
Glass
Arginine
Analysis of Variance
Cell Culture Techniques
Immunohistochemistry
Observation
Brain

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Basal nitric oxide production is enhanced by hydraulic pressure in cultured human trabecular cells. / Matsuo, Toshihiko.

In: British Journal of Ophthalmology, Vol. 84, No. 6, 06.2000, p. 631-635.

Research output: Contribution to journalArticle

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abstract = "Background/aims - Nitric oxide donors reduce intraocular pressure. Human trabecular cells in culture were examined for their nitric oxide production in response to hydraulic pressure. Methods - Human trabecular cells were cultured from trabeculum tissue fragments excised during trabeculectomy and exposed to hydraulic pressure change in a culture flask connected to a glass syringe. The pressure was exerted by automatic infusion of the piston of the syringe and monitored by a pressure gauge. The intracellular nitric oxide level was measured in real time with a nitric oxide binding fluorescent dye, diaminofluorescein-2. Results - Intracellular nitric oxide levels in cultured trabecular cells showed spontaneous fluctuation during 400 seconds of observation. Peak levels of intracellular nitric oxide were significantly higher at hydraulic pressure of 30, 40, and 50 mmHg, compared with 0 and 25 mmHg (p <0.0001, one way ANOVA, and p <0.05, Tukey-Kramer test). The fluctuation was completely abolished by the presence of N-methyl-L-arginine (L-NMMA), a nitric oxide synthase inhibitor. The cultured trabecular cells were shown by immunohistochemistry to express brain nitric oxide synthase (bNOS). Conclusion - Higher levels of hydraulic pressure enhanced basal production of nitric oxide in human trabecular cells. Nitric oxide would be a physiological mediator in the regulation of intraocular pressure.",
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