Barley yellow mosaic virus VPg is the determinant protein for breaking eIF4E-mediated recessive resistance in barley plants

Huangai Li, Hideki Kondo, Thomas Kühne, Yukio Shirako

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, we investigated the barley yellow mosaic virus (BaYMV, genus Bymovirus) factor(s) responsible for breaking eIF4E-mediated recessive resistance genes (rym4/5/6) in barley. Genome mapping analysis using chimeric infectious cDNA clones between rym5-breaking (JT10) and rym5-non-breaking (JK05) isolates indicated that genome-linked viral protein (VPg) is the determinant protein for breaking the rym5 resistance. Likewise, VPg is also responsible for overcoming the resistances of rym4 and rym6 alleles. Mutational analysis identified that amino acids Ser-118, Thr-120, and His-142 in JT10 VPg are the most critical residues for overcoming rym5 resistance in protoplasts. Moreover, the rym5-non-breaking JK05 could accumulate in the rym5 protoplasts when eIF4E derived from a susceptible barley cultivar was expressed from the viral genome. Thus, the compatibility between VPg and host eIF4E determines the ability of BaYMV to infect barley plants.

Original languageEnglish
Article number1449
JournalFrontiers in Plant Science
Volume7
Issue numberSeptember2016
DOIs
Publication statusPublished - Sep 30 2016

Fingerprint

Barley yellow mosaic virus
barley
protoplasts
Bymovirus
genome
proteins
viral proteins
chromosome mapping
clones
alleles
amino acids
cultivars
genes

Keywords

  • Barley yellow mosaic virus
  • eIF4E
  • Mesophyll protoplast
  • Resistance-breaking
  • Rym4/5/6
  • Virus movement
  • Virus replication
  • VPg

ASJC Scopus subject areas

  • Plant Science

Cite this

Barley yellow mosaic virus VPg is the determinant protein for breaking eIF4E-mediated recessive resistance in barley plants. / Li, Huangai; Kondo, Hideki; Kühne, Thomas; Shirako, Yukio.

In: Frontiers in Plant Science, Vol. 7, No. September2016, 1449, 30.09.2016.

Research output: Contribution to journalArticle

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