Barley NARROW LEAFED DWARF1 encoding a WUSCHEL-RELATED HOMEOBOX 3 (WOX3) regulates the marginal development of lateral organs

Takanori Yoshikawa, Shin Ya Tanaka, Yuuki Masumoto, Naoya Nobori, Hiroto Ishii, Ken Ichiro Hibara, Jun Ichi Itoh, Takatoshi Tanisaka, Shin Taketa

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7 Citations (Scopus)

Abstract

Barley (Hordeum vulgare L.) is the fourth most-produced cereal in the world and is mainly utilized as animal feed and malts. Recently barley attracts considerable attentions as healthy food rich in dietary fiber. However, limited knowledge is available about developmental aspects of barley leaves. In the present study, we investigated barley narrow leafed dwarf1 (nld1) mutants, which exhibit thin leaves accompanied by short stature. Detailed histological analysis revealed that leaf marginal tissues, such as sawtooth hairs and sclerenchymatous cells, were lacked in nld1, suggesting that narrowed leaf of nld1 was attributable to the defective development of the marginal regions in the leaves. The defective marginal developments were also appeared in internodes and glumes in spikelets. Map-based cloning revealed that NLD1 encodes a WUSCHEL-RELATED HOMEOBOX 3 (WOX3), an ortholog of the maize NARROW SHEATH genes. In situ hybridization showed that NLD1 transcripts were localized in the marginal edges of leaf primordia from the initiating stage. From these results, we concluded that NLD1 plays pivotal role in the increase of organ width and in the development of marginal tissues in lateral organs in barley.

Original languageEnglish
Pages (from-to)416-424
Number of pages9
JournalBreeding Science
Volume66
Issue number3
DOIs
Publication statusPublished - 2016

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Keywords

  • Barley
  • Hordeum vulgare L
  • Lateral organ
  • Marginal development
  • Narrow leafed dwarf1
  • WUSCHEL-RELATED HOMEOBOX 3

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Yoshikawa, T., Tanaka, S. Y., Masumoto, Y., Nobori, N., Ishii, H., Hibara, K. I., Itoh, J. I., Tanisaka, T., & Taketa, S. (2016). Barley NARROW LEAFED DWARF1 encoding a WUSCHEL-RELATED HOMEOBOX 3 (WOX3) regulates the marginal development of lateral organs. Breeding Science, 66(3), 416-424. https://doi.org/10.1270/jsbbs.16019