Bacteroides vulgatus and Bacteroides dorei Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis

Naofumi Yoshida, Takuo Emoto, Tomoya Yamashita, Hikaru Watanabe, Tomohiro Hayashi, Tokiko Tabata, Namiko Hoshi, Naoya Hatano, Genki Ozawa, Naoto Sasaki, Taiji Mizoguchi, Hilman Zulkifli Amin, Yushi Hirota, Wataru Ogawa, Takuji Yamada, Ken Ichi Hirata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: It is increasingly recognized that gut microbiota play a pivotal role in the development of atherosclerotic cardiovascular disease. Previously, we have reported that the abundance of genus Bacteroides is lower in patients with coronary artery disease (CAD) than in patients without CAD with coronary risk factors or in healthy volunteers. However, it remains unclear which and how specific gut bacteria contribute to the progression of atherosclerosis. METHODS: We recruited patients with CAD patients and controls without CAD with coronary risk factors. We then compared gut microbial composition using 16S ribosomal RNA gene sequencing in fecal samples to detect species with differential abundance between 2 groups. Subsequently, we used atherosclerosis-prone mice to study the mechanisms underlying the relationship between such species and atherosclerosis. RESULTS: Human fecal 16S ribosomal RNA gene sequencing revealed a significantly lower abundance of Bacteroides vulgatus and Bacteroides dorei in patients with CAD. This significant differential abundance was confirmed by quantitative polymerase chain reaction. Gavage with live B. vulgatus and B. dorei attenuated atherosclerotic lesion formation in atherosclerosis-prone mice, markedly ameliorating endotoxemia followed by decreasing gut microbial lipopolysaccharide production, effectively suppressing proinflammatory immune responses. Furthermore, fecal lipopolysaccharide levels in patients with CAD were significantly higher and negatively correlated with the abundance of B. vulgatus and B. dorei. CONCLUSIONS: Our translational research findings identify a previously unknown link between specific gut bacteria and atherosclerosis. Treatment with live B. vulgatus and B. dorei may help prevent CAD. CLINICAL TRIAL REGISTRATION: URL: https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000018051 . Unique identifier: UMIN000015703.

Original languageEnglish
Pages (from-to)2486-2498
Number of pages13
JournalCirculation
Volume138
Issue number22
DOIs
Publication statusPublished - Nov 27 2018
Externally publishedYes

Fingerprint

Bacteroides
Lipopolysaccharides
Coronary Artery Disease
Atherosclerosis
16S Ribosomal RNA
RNA Sequence Analysis
rRNA Genes
Bacteria
Endotoxemia
Translational Medical Research
Healthy Volunteers
Cardiovascular Diseases
Clinical Trials
Polymerase Chain Reaction

Keywords

  • Bacteroides dorei
  • Bacteroides vulgatus
  • coronary artery disease
  • gut microbiome
  • lipopolysaccharide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Yoshida, N., Emoto, T., Yamashita, T., Watanabe, H., Hayashi, T., Tabata, T., ... Hirata, K. I. (2018). Bacteroides vulgatus and Bacteroides dorei Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis. Circulation, 138(22), 2486-2498. https://doi.org/10.1161/CIRCULATIONAHA.118.033714

Bacteroides vulgatus and Bacteroides dorei Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis. / Yoshida, Naofumi; Emoto, Takuo; Yamashita, Tomoya; Watanabe, Hikaru; Hayashi, Tomohiro; Tabata, Tokiko; Hoshi, Namiko; Hatano, Naoya; Ozawa, Genki; Sasaki, Naoto; Mizoguchi, Taiji; Amin, Hilman Zulkifli; Hirota, Yushi; Ogawa, Wataru; Yamada, Takuji; Hirata, Ken Ichi.

In: Circulation, Vol. 138, No. 22, 27.11.2018, p. 2486-2498.

Research output: Contribution to journalArticle

Yoshida, N, Emoto, T, Yamashita, T, Watanabe, H, Hayashi, T, Tabata, T, Hoshi, N, Hatano, N, Ozawa, G, Sasaki, N, Mizoguchi, T, Amin, HZ, Hirota, Y, Ogawa, W, Yamada, T & Hirata, KI 2018, 'Bacteroides vulgatus and Bacteroides dorei Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis', Circulation, vol. 138, no. 22, pp. 2486-2498. https://doi.org/10.1161/CIRCULATIONAHA.118.033714
Yoshida, Naofumi ; Emoto, Takuo ; Yamashita, Tomoya ; Watanabe, Hikaru ; Hayashi, Tomohiro ; Tabata, Tokiko ; Hoshi, Namiko ; Hatano, Naoya ; Ozawa, Genki ; Sasaki, Naoto ; Mizoguchi, Taiji ; Amin, Hilman Zulkifli ; Hirota, Yushi ; Ogawa, Wataru ; Yamada, Takuji ; Hirata, Ken Ichi. / Bacteroides vulgatus and Bacteroides dorei Reduce Gut Microbial Lipopolysaccharide Production and Inhibit Atherosclerosis. In: Circulation. 2018 ; Vol. 138, No. 22. pp. 2486-2498.
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AU - Hayashi, Tomohiro

AU - Tabata, Tokiko

AU - Hoshi, Namiko

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AU - Ozawa, Genki

AU - Sasaki, Naoto

AU - Mizoguchi, Taiji

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