Bacterial lipopolysaccharide induces transforming growth factor β and hepatocyte growth factor through toll-like receptor 2 in cultured human colon cancer cells

T. Yoshioka, Y. Morimoto, H. Iwagaki, H. Itoh, S. Saito, N. Kobayashi, T. Yagi, N. Tanaka

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This study examined, in human cancer cell lines, the pattern of cytokine production stimulated by lipopolysaccharide (LPS), a major component of the outer surface of Gram-negative bacteria, and characterized the expression pattern of CD14, cell surface LPS receptor antigen, and toll-like receptors (TLRs), which appear to be key regulators of the innate immune response system. Two colon cancer cell lines (DLD and LoVo), a hepatocellular carcinoma cell line and a myelomonocytic cell line were incubated with LPS for 0 - 72 h, and transforming growth factor (TGF) β1 and β2, hepatocyte growth factor (HGF) and interleukins 6, 8 and 15 were assayed. The only changes induced by incubation with LPS were significant increases in TGFβ1 production at 12 h, and in HGF production at 72 h, in LPS-stimulated DLD cells, and significant increases in TGFβ2 production after 12 h and in HGF after 72 h in LoVo cells. Using reverse transcriptase-polymerase chain reaction analysis, expression of CD14 and TLR-2 mRNA was detected in DLD and LoVo cells, and expression of TLR-4 mRNA was detected in PLC/PRF/5 and KG-1 cells. These results suggest that LPS induces TGFβ and HGF production mediated by CD14/TLR-2 in cultured human colon cancer cell lines.

Original languageEnglish
Pages (from-to)409-420
Number of pages12
JournalJournal of International Medical Research
Issue number5
Publication statusPublished - Jan 1 2001



  • Hepatocyte growth factor
  • Lipopolysaccharide
  • Toll-like receptor
  • Transforming growth factor

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biochemistry, medical

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