Bacterial DNA activates immunity in Arabidopsis thaliana

Suguru Yakushiji, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that non-methylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalJournal of General Plant Pathology
Volume75
Issue number3
DOIs
Publication statusPublished - Jun 1 2009

Keywords

  • Arabidopsis thaliana
  • Bacterial DNA
  • CpG DNA
  • Endocytosis
  • MAMP
  • Non-host resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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