Bacterial DNA activates immunity in Arabidopsis thaliana

Suguru Yakushiji, Yasuhiro Ishiga, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plant Arabidopsis thaliana was examined. EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereas SmaI- and HapII-digested plasmid DNA and EcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA and Escherichia coli DNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that non-methylated CpG DNA, as a MAMP, induced defense responses in Arabidopsis and that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalJournal of General Plant Pathology
Volume75
Issue number3
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Arabidopsis thaliana
immunity
DNA
plasmids
endocytosis
microorganisms
callose
herring
microbial activity
methylation
reactive oxygen species
cytoplasm
Arabidopsis
Escherichia coli
organisms
animals

Keywords

  • Arabidopsis thaliana
  • Bacterial DNA
  • CpG DNA
  • Endocytosis
  • MAMP
  • Non-host resistance

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Bacterial DNA activates immunity in Arabidopsis thaliana. / Yakushiji, Suguru; Ishiga, Yasuhiro; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Journal of General Plant Pathology, Vol. 75, No. 3, 06.2009, p. 227-234.

Research output: Contribution to journalArticle

Yakushiji, Suguru ; Ishiga, Yasuhiro ; Inagaki, Yoshishige ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Ichinose, Yuki. / Bacterial DNA activates immunity in Arabidopsis thaliana. In: Journal of General Plant Pathology. 2009 ; Vol. 75, No. 3. pp. 227-234.
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