Bacterial collagen-binding domain targets undertwisted regions of collagen

Sagaya Theresa Leena Philominathan, Takaki Koide, Osamu Matsushita, Joshua Sakon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Clostridium histolyticum collagenase causes extensive degradation of collagen in connective tissue that results in gas gangrene. The C-terminal collagen-binding domain (CBD) of these enzymes is the minimal segment required to bind to a collagen fibril. CBD binds unidirectionally to the undertwisted C-terminus of triple helical collagen. Here, we examine whether CBD could also target undertwisted regions even in the middle of the triple helix. Collageneous peptides with an additional undertwisted region were synthesized by introducing a Gly → Ala substitution [(POG)xPOA(POG)y] 3, where x + y = 9 and x > 3). 1H-15N heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR) titration studies with 15N-labeled CBD demonstrated that the minicollagen binds to a 10 Å wide 25 Å long cleft. Six collagenous peptides each labeled with a nitroxide radical were then titrated with 15N-labeled CBD. CBD binds to either the Gly → Ala substitution site or to the C-terminus of each minicollagen. Small-angle X-ray scattering measurements revealed that CBD prefers to bind the Gly → Ala site to the C-terminus. The HSQC NMR spectra of 15N-labeled minicollagen and minicollagen with undertwisted regions were unaffected by the titration of unlabeled CBD. The results imply that CBD binds to the undertwisted region of the minicollagen but does not actively unwind the triple helix.

Original languageEnglish
Pages (from-to)1554-1565
Number of pages12
JournalProtein Science
Volume21
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Collagen
Titration
Microbial Collagenase
Substitution reactions
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Gas Gangrene
Peptides
X ray scattering
Connective Tissue
Gases
X-Rays
Tissue
Degradation
Enzymes

Keywords

  • Binding
  • Collagen
  • NMR
  • Triple-helix
  • Undertwisted collagen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Bacterial collagen-binding domain targets undertwisted regions of collagen. / Philominathan, Sagaya Theresa Leena; Koide, Takaki; Matsushita, Osamu; Sakon, Joshua.

In: Protein Science, Vol. 21, No. 10, 10.2012, p. 1554-1565.

Research output: Contribution to journalArticle

Philominathan, Sagaya Theresa Leena ; Koide, Takaki ; Matsushita, Osamu ; Sakon, Joshua. / Bacterial collagen-binding domain targets undertwisted regions of collagen. In: Protein Science. 2012 ; Vol. 21, No. 10. pp. 1554-1565.
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