Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters Thryonomys swinderianus in Ghana

Christopher Adenyo, Kenji Ohya, Yongjin Qiu, Yasuhiro Takashima, Hirohito Ogawa, Tateki Matsumoto, May June Thu, Kozue Sato, Hiroki Kawabata, Yukie Katayama, Tsutomu Omatsu, Tetsuya Mizutani, Hideto Fukushi, Ken Katakura, Narikaki Nonaka, Miho Inoue-Murayama, Kayang Boniface, Ryo Nakao

Research output: Contribution to journalArticle

Abstract

Ticks and tick-borne pathogens constitute a great threat to livestock production and are a potential health hazard to humans. Grasscutters (Thryonomys swinderianus) are widely hunted for meat in Ghana and many other West and Central African countries. However, tick-borne zoonotic risks posed by wild grasscutters have not been assessed. The objective of this study was to investigate bacterial and protozoan pathogens in ticks infecting wild grasscutters. A total of 81 ticks were collected from three hunted grasscutters purchased from Katamanto, the central bushmeat market in Accra. Ticks were identified as Ixodes aulacodi and Rhipicephalus sp. based on morphological keys, which were further confirmed by sequencing mitochondrial 16S ribosomal DNA (rDNA) and cytochrome oxidase I (COI) genes of specimens. Protozoan infections were tested by PCR amplifying 18S rDNA of Babesia/Theileria/Hepatozoon, while bacterial infections were evaluated by PCRs or real-time PCRs targeting Anaplasmataceae, Borrelia, spotted fever group rickettsiae, chlamydiae and Candidatus Midichloria mitochondrii. The results of PCR screening showed that 35.5% (27 out of 76) of I. aulacodi were positive for parasite infections. Sequencing analysis of the amplified products gave one identical sequence showing similarity with Babesia spp. reported from Africa. The Ca. M. mitochondrii endosymbiont was present in 85.5% (65 out of 76) of I. aulacodi but not in the five Rhipicephalus ticks. Two Anaplasmataceae bacteria genetically related to Ehrlichia muris and Anaeplasma phagocytophilum were also detected in two I. aulacodi. None of the ticks were positive for Borrelia spp., spotted fever group rickettsiae and chlamydiae. Since I. aulacodi on wild grasscutters are potential carriers of tick-borne pathogens, some of which could be of zoonotic potential, rigorous tick control and pathogen analyses should be instituted especially when wild caught grasscutters are being used as foundation stock for breedings.

Original languageEnglish
Pages (from-to)105388
JournalActa Tropica
DOIs
Publication statusE-pub ahead of print - Feb 5 2020
Externally publishedYes

Fingerprint

Thryonomys
Ghana
Ticks
symbionts
Protozoa
ticks
pathogens
Anaplasmataceae
Rhipicephalus
Borrelia
Babesia
Rickettsia
Chlamydia
Zoonoses
Ribosomal DNA
ribosomal DNA
Polymerase Chain Reaction
fever
Ehrlichia muris
Fever

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Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters Thryonomys swinderianus in Ghana. / Adenyo, Christopher; Ohya, Kenji; Qiu, Yongjin; Takashima, Yasuhiro; Ogawa, Hirohito; Matsumoto, Tateki; Thu, May June; Sato, Kozue; Kawabata, Hiroki; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya; Fukushi, Hideto; Katakura, Ken; Nonaka, Narikaki; Inoue-Murayama, Miho; Boniface, Kayang; Nakao, Ryo.

In: Acta Tropica, 05.02.2020, p. 105388.

Research output: Contribution to journalArticle

Adenyo, C, Ohya, K, Qiu, Y, Takashima, Y, Ogawa, H, Matsumoto, T, Thu, MJ, Sato, K, Kawabata, H, Katayama, Y, Omatsu, T, Mizutani, T, Fukushi, H, Katakura, K, Nonaka, N, Inoue-Murayama, M, Boniface, K & Nakao, R 2020, 'Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters Thryonomys swinderianus in Ghana', Acta Tropica, pp. 105388. https://doi.org/10.1016/j.actatropica.2020.105388
Adenyo, Christopher ; Ohya, Kenji ; Qiu, Yongjin ; Takashima, Yasuhiro ; Ogawa, Hirohito ; Matsumoto, Tateki ; Thu, May June ; Sato, Kozue ; Kawabata, Hiroki ; Katayama, Yukie ; Omatsu, Tsutomu ; Mizutani, Tetsuya ; Fukushi, Hideto ; Katakura, Ken ; Nonaka, Narikaki ; Inoue-Murayama, Miho ; Boniface, Kayang ; Nakao, Ryo. / Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters Thryonomys swinderianus in Ghana. In: Acta Tropica. 2020 ; pp. 105388.
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AU - Qiu, Yongjin

AU - Takashima, Yasuhiro

AU - Ogawa, Hirohito

AU - Matsumoto, Tateki

AU - Thu, May June

AU - Sato, Kozue

AU - Kawabata, Hiroki

AU - Katayama, Yukie

AU - Omatsu, Tsutomu

AU - Mizutani, Tetsuya

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AU - Nonaka, Narikaki

AU - Inoue-Murayama, Miho

AU - Boniface, Kayang

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