Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique

Yosef Hamba Tola, Yoshiko Fujitani, Akio Tani

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Motile bacteria often exhibit chemotaxis toward favorable compounds. However, the diversity of bacteria that are attracted to a given substance is largely unknown. This study aimed to reveal the diversity of bacteria with natural chemotaxis towards methanol. We tried to enrich environmental chemotactic bacteria using a glass capillary that is half-filled with methanol solidified with agarose as a trap (“chemotaxis fishing”). The pilot experiment using methanol-chemotactic Methylobacterium aquaticum strain 22A enriched the cells by 46-fold. The method was then applied to bacterial suspensions from paddy water and plants. Depending on the isolation sources and the methods of motility induction, methylotrophic bacteria were enriched 1.2–330-fold. The fished isolates belong to 32 species in 18 genera, mainly containing Acinetobacter, Methylobacterium and Pseudomonas species. Our chemotaxis fishing unveiled a part of diversity of the bacteria with natural chemotaxis towards methanol.

    Original languageEnglish
    Pages (from-to)2163-2171
    Number of pages9
    JournalBioscience, Biotechnology and Biochemistry
    Volume83
    Issue number11
    DOIs
    Publication statusPublished - 2019

    Keywords

    • Chemotaxis
    • Methanol
    • Methylobacterium
    • Plant-associated bacteria
    • Whole-cell MALDI-TOF/MS

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

    Fingerprint

    Dive into the research topics of 'Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique'. Together they form a unique fingerprint.

    Cite this