Bacillus hisashii sp. Nov., isolated from the caeca of gnotobiotic mice fed with thermophilefermented compost

Ayaka Nishida, Hirokuni Miyamoto, Sankichi Horiuchi, Ryo Watanabe, Hidetoshi Morita, Shinji Fukuda, Hiroshi Ohno, Shizuko Ichinose, Hisashi Miyamoto, Hiroaki Kodama

Research output: Contribution to journalArticle

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Abstract

A taxonomic study was performed on 15 bacterial isolates from the caeca of gnotobiotic mice that had been fed with thermophile-fermented compost. The 15 isolates were thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming bacteria, and were most closely related to Bacillus thermoamylovorans CNCM I-1378T. The 16S rRNA gene sequence of strain N-11T, selected as representative of this new group, showed a similarity of 99.4% with Bacillus thermoamylovorans CNCM I-1378T, 94.7% with Bacillus thermolactis R-6488T, and 94.4% with Bacillus kokeshiiformis MO-04T. The isolates were then classified into two distinct groups based on a (GTG)5-fingerprint analysis. Two isolates, N-11T and N-21, were the representatives of these two groups, respectively.‘The N-11T and N-21 isolates showed 66–71% DNA–DNA relatedness with one other, but had less than 37% DNA–DNA relatedness with B. thermoamylovorans LMG 18084T. The other 13 isolates showed DNA–DNA relatedness values above 74% with the N-11T isolate. All 15 isolates grew at 25–60 °C (optimum 50 °C), pH 6–8 (optimum pH 7) and were capable of growing on a medium containing 6% (w/v) NaCl (optimum 0.5%). The 15 isolates could be distinguished from B. thermoamylovorans LMG 18084T because they showed Tween 80 hydrolysis activity and did not produce acid from melibiose. The major fatty acids were anteiso-C15:0, C16:0, iso-C15:0, iso-C14:0 and iso-C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and several unidentified phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The menaquinone was MK-7. The DNA G+C content was 37.9 mol%. Based on the phenotypic properties, the 15 strains represent a novel species of the genus Bacillus, for which the name Bacillus hisashii sp. nov. is proposed. The type strain is N-11T (=NRBC 110226T=LMG 28201T).

Original languageEnglish
Pages (from-to)3944-3949
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number11
DOIs
Publication statusPublished - Nov 1 2015

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Bacillus thermoamylovorans
Germ-Free Life
Bacillus (bacteria)
relatedness
cecum
compost
Bacillus
composts
Soil
acid
mice
acids
thermophilic microorganisms
melibiose
phospholipid
endospores
cardiolipins
Endospore-Forming Bacteria
hydrolysis
menaquinones

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Bacillus hisashii sp. Nov., isolated from the caeca of gnotobiotic mice fed with thermophilefermented compost. / Nishida, Ayaka; Miyamoto, Hirokuni; Horiuchi, Sankichi; Watanabe, Ryo; Morita, Hidetoshi; Fukuda, Shinji; Ohno, Hiroshi; Ichinose, Shizuko; Miyamoto, Hisashi; Kodama, Hiroaki.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 11, 01.11.2015, p. 3944-3949.

Research output: Contribution to journalArticle

Nishida, A, Miyamoto, H, Horiuchi, S, Watanabe, R, Morita, H, Fukuda, S, Ohno, H, Ichinose, S, Miyamoto, H & Kodama, H 2015, 'Bacillus hisashii sp. Nov., isolated from the caeca of gnotobiotic mice fed with thermophilefermented compost', International Journal of Systematic and Evolutionary Microbiology, vol. 65, no. 11, pp. 3944-3949. https://doi.org/10.1099/ijsem.0.000516
Nishida, Ayaka ; Miyamoto, Hirokuni ; Horiuchi, Sankichi ; Watanabe, Ryo ; Morita, Hidetoshi ; Fukuda, Shinji ; Ohno, Hiroshi ; Ichinose, Shizuko ; Miyamoto, Hisashi ; Kodama, Hiroaki. / Bacillus hisashii sp. Nov., isolated from the caeca of gnotobiotic mice fed with thermophilefermented compost. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 11. pp. 3944-3949.
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