Auxin transport sites are visualized in planta using fluorescent auxin analogs

Ken Ichiro Hayashi, Shouichi Nakamura, Shiho Fukunaga, Takeshi Nishimura, Mark K. Jenness, Angus S. Murphy, Hiroyasu Motose, Hiroshi Nozaki, Masahiko Furutani, Takashi Aoyama

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The plant hormone auxin is a key morphogenetic signal that controls many aspects of plant growth and development. Cellular auxin levels are coordinately regulated by multiple processes, including auxin biosynthesis and the polar transport and metabolic pathways. The auxin concentration gradient determines plant organ positioning and growth responses to environmental cues. Auxin transport systems play crucial roles in the spatiotemporal regulation of the auxin gradient. This auxin gradient has been analyzed using SCF-type E3 ubiquitin-ligase complex-based auxin biosensors in synthetic auxin-responsive reporter lines. However, the contributions of auxin biosynthesis and metabolism to the auxin gradient have been largely elusive. Additionally, the available information on subcellular auxin localization is still limited. Here we designed fluorescently labeled auxin analogs that remain active for auxin transport but are inactive for auxin signaling and metabolism. Fluorescent auxin analogs enable the selective visualization of the distribution of auxin by the auxin transport system. Together with auxin biosynthesis inhibitors and an auxin biosensor, these analogs indicated a substantial contribution of local auxin biosynthesis to the formation of auxin maxima at the root apex. Moreover, fluorescent auxin analogs mainly localized to the endoplasmic reticulum in cultured cells and roots, implying the presence of a subcellular auxin gradient in the cells. Our work not only provides a useful tool for the plant chemical biology field but also demonstrates a new strategy for imaging the distribution of small-molecule hormones.

Original languageEnglish
Pages (from-to)11557-11562
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number31
DOIs
Publication statusPublished - Aug 5 2014

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Keywords

  • Auxin transporter
  • Subcellular localization

ASJC Scopus subject areas

  • General

Cite this

Hayashi, K. I., Nakamura, S., Fukunaga, S., Nishimura, T., Jenness, M. K., Murphy, A. S., ... Aoyama, T. (2014). Auxin transport sites are visualized in planta using fluorescent auxin analogs. Proceedings of the National Academy of Sciences of the United States of America, 111(31), 11557-11562. https://doi.org/10.1073/pnas.1408960111

Auxin transport sites are visualized in planta using fluorescent auxin analogs. / Hayashi, Ken Ichiro; Nakamura, Shouichi; Fukunaga, Shiho; Nishimura, Takeshi; Jenness, Mark K.; Murphy, Angus S.; Motose, Hiroyasu; Nozaki, Hiroshi; Furutani, Masahiko; Aoyama, Takashi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 31, 05.08.2014, p. 11557-11562.

Research output: Contribution to journalArticle

Hayashi, KI, Nakamura, S, Fukunaga, S, Nishimura, T, Jenness, MK, Murphy, AS, Motose, H, Nozaki, H, Furutani, M & Aoyama, T 2014, 'Auxin transport sites are visualized in planta using fluorescent auxin analogs', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 31, pp. 11557-11562. https://doi.org/10.1073/pnas.1408960111
Hayashi, Ken Ichiro ; Nakamura, Shouichi ; Fukunaga, Shiho ; Nishimura, Takeshi ; Jenness, Mark K. ; Murphy, Angus S. ; Motose, Hiroyasu ; Nozaki, Hiroshi ; Furutani, Masahiko ; Aoyama, Takashi. / Auxin transport sites are visualized in planta using fluorescent auxin analogs. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 31. pp. 11557-11562.
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