Autotaxin controls caudal diencephalon-mesencephalon development in the chick

Hideyo Ohuchi, Hitomi Fukui, Akane Matsuyo, Sayuri Tomonari, Masayuki Tanaka, Hiroyuki Arai, Sumihare Noji, Junken Aoki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The diencephalon is the embryonic anlagen of the higher integration centers of the brain. Recent studies have elucidated how the cells in the rostral diencephalon acquire their regional identities. However, the understanding of the mechanisms under which the caudal diencephalon is formed is still limited. Here we focus on the role of Autotaxin (ATX), a lysophospholipid-generating exoenzyme, whose mRNA is detected in the caudal diencephalon. RNA interference against ATX altered the expression pattern of Pax6-regualted genes, Tcf4, Lim1, and En1, implying that ATX is required for the maintenance of the regional identity of the caudal diencephalon and the diencephalon-mesencephalon boundary (DMB). Furthermore, ATX-RNAi inhibited neuroepithelial cell proliferation on both sides of the DMB. We propose a dual role of ATX in chick brain development, in which ATX not only contributes to the formation of caudal diencephalon as a short-range signal, but also regulates the growth of mesencephalon as a long-range signal.

Original languageEnglish
Pages (from-to)2647-2658
Number of pages12
JournalDevelopmental Dynamics
Volume239
Issue number10
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Diencephalon
Mesencephalon
RNA Interference
Neuroepithelial Cells
Lysophospholipids
Brain
Maintenance
Cell Proliferation
Messenger RNA
Growth

Keywords

  • Autotaxin
  • Brain development
  • Caudal diencephalon
  • Chick
  • ENPP2
  • Lim1
  • Mesencephalon
  • Pax6

ASJC Scopus subject areas

  • Developmental Biology
  • Medicine(all)

Cite this

Ohuchi, H., Fukui, H., Matsuyo, A., Tomonari, S., Tanaka, M., Arai, H., ... Aoki, J. (2010). Autotaxin controls caudal diencephalon-mesencephalon development in the chick. Developmental Dynamics, 239(10), 2647-2658. https://doi.org/10.1002/dvdy.22403

Autotaxin controls caudal diencephalon-mesencephalon development in the chick. / Ohuchi, Hideyo; Fukui, Hitomi; Matsuyo, Akane; Tomonari, Sayuri; Tanaka, Masayuki; Arai, Hiroyuki; Noji, Sumihare; Aoki, Junken.

In: Developmental Dynamics, Vol. 239, No. 10, 10.2010, p. 2647-2658.

Research output: Contribution to journalArticle

Ohuchi, H, Fukui, H, Matsuyo, A, Tomonari, S, Tanaka, M, Arai, H, Noji, S & Aoki, J 2010, 'Autotaxin controls caudal diencephalon-mesencephalon development in the chick', Developmental Dynamics, vol. 239, no. 10, pp. 2647-2658. https://doi.org/10.1002/dvdy.22403
Ohuchi, Hideyo ; Fukui, Hitomi ; Matsuyo, Akane ; Tomonari, Sayuri ; Tanaka, Masayuki ; Arai, Hiroyuki ; Noji, Sumihare ; Aoki, Junken. / Autotaxin controls caudal diencephalon-mesencephalon development in the chick. In: Developmental Dynamics. 2010 ; Vol. 239, No. 10. pp. 2647-2658.
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