Autoradiographic study of [3H]flunitrazepam binding sites in the subnuclei of the thalamus of rats rendered tolerant to and dependent on pentobarbital

Toshihito Suzuki, Takehiko Ito, Susan E. Wellman, Ing K. Ho

Research output: Contribution to journalArticle

Abstract

We examined changes in benzodiazepine binding sites labeled by [3H]flunitrazepam in five nuclei of the thalamus, the central medial, central lateral, intermediodorsal, ventroposterior, and laterodorsal nuclei, in rats made tolerant to and dependent on pentobarbital. Animals were made tolerant by intracerebroventricular infusion with pentobarbital (300 μg (10 μl)-1 h-1 for six days) through pre-implanted cannulae. Pentobarbital dependence was assessed 24 h after abrupt withdrawal from pentobarbital. Pentobarbital-tolerant rats showed no significant change in [3H]flunitrazepam binding sites (B(max) and K(d)) in any nucleus examined in the thalamus. In the rats made dependent on pentobarbital, significant increases in the B(max) of [3H]flunitrazepam binding without changes in K(d) were noted in central medial and central lateral nuclei. GABAergic (γ-aminobutyric acid) neurons in the ventrobasal nucleus and in nuclei in the midline group are important in seizure regulation and arousal. These findings suggest that alterations of benzodiazepine receptors in certain nuclei of thalami are involved in the physiological changes induced by pentobarbital dependence. There were no changes in the binding parameters for [3H]flunitrazepam in pentobarbital-tolerant rats. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalEuropean Journal of Pharmacology
Volume354
Issue number2-3
DOIs
Publication statusPublished - Aug 7 1998

Fingerprint

Flunitrazepam
Pentobarbital
Thalamus
Binding Sites
Intralaminar Thalamic Nuclei
Intraventricular Infusions
Aminobutyrates
GABA-A Receptors
Arousal
Benzodiazepines
Seizures
Neurons

Keywords

  • Benzodiazepine
  • Dependence
  • Pentobarbital
  • Thalamus
  • Tolerance

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Autoradiographic study of [3H]flunitrazepam binding sites in the subnuclei of the thalamus of rats rendered tolerant to and dependent on pentobarbital. / Suzuki, Toshihito; Ito, Takehiko; Wellman, Susan E.; Ho, Ing K.

In: European Journal of Pharmacology, Vol. 354, No. 2-3, 07.08.1998, p. 145-151.

Research output: Contribution to journalArticle

@article{2644bb03f8674f1e976cd3dd4baeaa8e,
title = "Autoradiographic study of [3H]flunitrazepam binding sites in the subnuclei of the thalamus of rats rendered tolerant to and dependent on pentobarbital",
abstract = "We examined changes in benzodiazepine binding sites labeled by [3H]flunitrazepam in five nuclei of the thalamus, the central medial, central lateral, intermediodorsal, ventroposterior, and laterodorsal nuclei, in rats made tolerant to and dependent on pentobarbital. Animals were made tolerant by intracerebroventricular infusion with pentobarbital (300 μg (10 μl)-1 h-1 for six days) through pre-implanted cannulae. Pentobarbital dependence was assessed 24 h after abrupt withdrawal from pentobarbital. Pentobarbital-tolerant rats showed no significant change in [3H]flunitrazepam binding sites (B(max) and K(d)) in any nucleus examined in the thalamus. In the rats made dependent on pentobarbital, significant increases in the B(max) of [3H]flunitrazepam binding without changes in K(d) were noted in central medial and central lateral nuclei. GABAergic (γ-aminobutyric acid) neurons in the ventrobasal nucleus and in nuclei in the midline group are important in seizure regulation and arousal. These findings suggest that alterations of benzodiazepine receptors in certain nuclei of thalami are involved in the physiological changes induced by pentobarbital dependence. There were no changes in the binding parameters for [3H]flunitrazepam in pentobarbital-tolerant rats. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Benzodiazepine, Dependence, Pentobarbital, Thalamus, Tolerance",
author = "Toshihito Suzuki and Takehiko Ito and Wellman, {Susan E.} and Ho, {Ing K.}",
year = "1998",
month = "8",
day = "7",
doi = "10.1016/S0014-2999(98)00447-6",
language = "English",
volume = "354",
pages = "145--151",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Autoradiographic study of [3H]flunitrazepam binding sites in the subnuclei of the thalamus of rats rendered tolerant to and dependent on pentobarbital

AU - Suzuki, Toshihito

AU - Ito, Takehiko

AU - Wellman, Susan E.

AU - Ho, Ing K.

PY - 1998/8/7

Y1 - 1998/8/7

N2 - We examined changes in benzodiazepine binding sites labeled by [3H]flunitrazepam in five nuclei of the thalamus, the central medial, central lateral, intermediodorsal, ventroposterior, and laterodorsal nuclei, in rats made tolerant to and dependent on pentobarbital. Animals were made tolerant by intracerebroventricular infusion with pentobarbital (300 μg (10 μl)-1 h-1 for six days) through pre-implanted cannulae. Pentobarbital dependence was assessed 24 h after abrupt withdrawal from pentobarbital. Pentobarbital-tolerant rats showed no significant change in [3H]flunitrazepam binding sites (B(max) and K(d)) in any nucleus examined in the thalamus. In the rats made dependent on pentobarbital, significant increases in the B(max) of [3H]flunitrazepam binding without changes in K(d) were noted in central medial and central lateral nuclei. GABAergic (γ-aminobutyric acid) neurons in the ventrobasal nucleus and in nuclei in the midline group are important in seizure regulation and arousal. These findings suggest that alterations of benzodiazepine receptors in certain nuclei of thalami are involved in the physiological changes induced by pentobarbital dependence. There were no changes in the binding parameters for [3H]flunitrazepam in pentobarbital-tolerant rats. Copyright (C) 1998 Elsevier Science B.V.

AB - We examined changes in benzodiazepine binding sites labeled by [3H]flunitrazepam in five nuclei of the thalamus, the central medial, central lateral, intermediodorsal, ventroposterior, and laterodorsal nuclei, in rats made tolerant to and dependent on pentobarbital. Animals were made tolerant by intracerebroventricular infusion with pentobarbital (300 μg (10 μl)-1 h-1 for six days) through pre-implanted cannulae. Pentobarbital dependence was assessed 24 h after abrupt withdrawal from pentobarbital. Pentobarbital-tolerant rats showed no significant change in [3H]flunitrazepam binding sites (B(max) and K(d)) in any nucleus examined in the thalamus. In the rats made dependent on pentobarbital, significant increases in the B(max) of [3H]flunitrazepam binding without changes in K(d) were noted in central medial and central lateral nuclei. GABAergic (γ-aminobutyric acid) neurons in the ventrobasal nucleus and in nuclei in the midline group are important in seizure regulation and arousal. These findings suggest that alterations of benzodiazepine receptors in certain nuclei of thalami are involved in the physiological changes induced by pentobarbital dependence. There were no changes in the binding parameters for [3H]flunitrazepam in pentobarbital-tolerant rats. Copyright (C) 1998 Elsevier Science B.V.

KW - Benzodiazepine

KW - Dependence

KW - Pentobarbital

KW - Thalamus

KW - Tolerance

UR - http://www.scopus.com/inward/record.url?scp=0031595412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031595412&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(98)00447-6

DO - 10.1016/S0014-2999(98)00447-6

M3 - Article

C2 - 9754914

AN - SCOPUS:0031595412

VL - 354

SP - 145

EP - 151

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2-3

ER -