Automated on-line in-tube solid-phase microextraction coupled with high performance liquid chromatography for the analysis of bisphenol A, alkylphenols, and phthalate esters in foods contacted with plastics

Hiroyuki Kataoka, Megumi Ise, Shizuo Narimatsu

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

An automated on-line method for the analysis of endocrine disruptors related to plastics was developed using in-tube solid-phase microextraction coupled with high performance liquid chromatography (in-tube SPME/HPLC). In-tube SPME is an extraction technique for organic compounds in aqueous samples, in which analytes are extracted from the sample directly into an open tubular capillary by repeated draw/eject cycles of sample solution. Suspected endocrine disrupting compounds, bisphenol A, two alkylphenols, and nine phthalate esters, tested in this study were well separated with a Hypersil ODS column with diode array detection using acetonitrile/water as a mobile phase. In order to optimize the extraction of these compounds, several in-tube SPME parameters were examined. The optimum extraction conditions were 20 draw/eject cycles of 40 μL of sample using a Supel-Q PLOT capillary column. The extracted compounds were easily desorbed from the capillary by mobile phase flow, and carryover was not observed. Using in-tube SPME/HPLC method, the calibration curves of these compounds were linear in the range from 1 to 500 ng/mL, with correlation coefficient above 0.991 (n = 18), and the detection limits (S/N = 3) were 0.1-4.0 ng/mL. This method was successfully applied to the migration test from plastics and the analysis of food samples.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalJournal of Separation Science
Volume25
Issue number1-2
DOIs
Publication statusPublished - 2002

Fingerprint

Solid Phase Microextraction
High performance liquid chromatography
Plastics
Esters
High Pressure Liquid Chromatography
Food
Food Analysis
Acetonitrile
Organic compounds
Endocrine Disruptors
Diodes
Calibration
Limit of Detection
phthalic acid
4-sulfophenylmethallyl ether
bisphenol A
Water

Keywords

  • Automated sample preparation
  • Endocrine disruptors
  • In-tube solid-phase microextraction
  • Phenols
  • Phthalate esters

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

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title = "Automated on-line in-tube solid-phase microextraction coupled with high performance liquid chromatography for the analysis of bisphenol A, alkylphenols, and phthalate esters in foods contacted with plastics",
abstract = "An automated on-line method for the analysis of endocrine disruptors related to plastics was developed using in-tube solid-phase microextraction coupled with high performance liquid chromatography (in-tube SPME/HPLC). In-tube SPME is an extraction technique for organic compounds in aqueous samples, in which analytes are extracted from the sample directly into an open tubular capillary by repeated draw/eject cycles of sample solution. Suspected endocrine disrupting compounds, bisphenol A, two alkylphenols, and nine phthalate esters, tested in this study were well separated with a Hypersil ODS column with diode array detection using acetonitrile/water as a mobile phase. In order to optimize the extraction of these compounds, several in-tube SPME parameters were examined. The optimum extraction conditions were 20 draw/eject cycles of 40 μL of sample using a Supel-Q PLOT capillary column. The extracted compounds were easily desorbed from the capillary by mobile phase flow, and carryover was not observed. Using in-tube SPME/HPLC method, the calibration curves of these compounds were linear in the range from 1 to 500 ng/mL, with correlation coefficient above 0.991 (n = 18), and the detection limits (S/N = 3) were 0.1-4.0 ng/mL. This method was successfully applied to the migration test from plastics and the analysis of food samples.",
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author = "Hiroyuki Kataoka and Megumi Ise and Shizuo Narimatsu",
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T1 - Automated on-line in-tube solid-phase microextraction coupled with high performance liquid chromatography for the analysis of bisphenol A, alkylphenols, and phthalate esters in foods contacted with plastics

AU - Kataoka, Hiroyuki

AU - Ise, Megumi

AU - Narimatsu, Shizuo

PY - 2002

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N2 - An automated on-line method for the analysis of endocrine disruptors related to plastics was developed using in-tube solid-phase microextraction coupled with high performance liquid chromatography (in-tube SPME/HPLC). In-tube SPME is an extraction technique for organic compounds in aqueous samples, in which analytes are extracted from the sample directly into an open tubular capillary by repeated draw/eject cycles of sample solution. Suspected endocrine disrupting compounds, bisphenol A, two alkylphenols, and nine phthalate esters, tested in this study were well separated with a Hypersil ODS column with diode array detection using acetonitrile/water as a mobile phase. In order to optimize the extraction of these compounds, several in-tube SPME parameters were examined. The optimum extraction conditions were 20 draw/eject cycles of 40 μL of sample using a Supel-Q PLOT capillary column. The extracted compounds were easily desorbed from the capillary by mobile phase flow, and carryover was not observed. Using in-tube SPME/HPLC method, the calibration curves of these compounds were linear in the range from 1 to 500 ng/mL, with correlation coefficient above 0.991 (n = 18), and the detection limits (S/N = 3) were 0.1-4.0 ng/mL. This method was successfully applied to the migration test from plastics and the analysis of food samples.

AB - An automated on-line method for the analysis of endocrine disruptors related to plastics was developed using in-tube solid-phase microextraction coupled with high performance liquid chromatography (in-tube SPME/HPLC). In-tube SPME is an extraction technique for organic compounds in aqueous samples, in which analytes are extracted from the sample directly into an open tubular capillary by repeated draw/eject cycles of sample solution. Suspected endocrine disrupting compounds, bisphenol A, two alkylphenols, and nine phthalate esters, tested in this study were well separated with a Hypersil ODS column with diode array detection using acetonitrile/water as a mobile phase. In order to optimize the extraction of these compounds, several in-tube SPME parameters were examined. The optimum extraction conditions were 20 draw/eject cycles of 40 μL of sample using a Supel-Q PLOT capillary column. The extracted compounds were easily desorbed from the capillary by mobile phase flow, and carryover was not observed. Using in-tube SPME/HPLC method, the calibration curves of these compounds were linear in the range from 1 to 500 ng/mL, with correlation coefficient above 0.991 (n = 18), and the detection limits (S/N = 3) were 0.1-4.0 ng/mL. This method was successfully applied to the migration test from plastics and the analysis of food samples.

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