Automated drug screening system for ion channel proteins

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, H. Sasaki, K. Kamiya, N. Miki, T. Ide, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes an automated drug screening system for Ca 2+-activated K+ channel proteins (BK channel). BK channels are pharmacological targets for the treatment of stroke. We have been developing multiple lipid bilayer membranes (BLMs) system for reconstituting the membrane proteins [1]. In this study, the osmotic pressure in the proteoliposomes was controlled to induce the vesicle fusion. As a result, we succeeded in simultaneous reconstitution of BK channels in the BLMs array using an injection robot (Fig. 1). Moreover, the channel inhibition behavior was examined using a K+ channel blocker. Since our method is applicable for multi-type of proteoliposomes, we believe that the system can be used as a high-throughput screening (HTS) system for membrane-protein-based drug discovery.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages76-78
Number of pages3
Publication statusPublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • BK channels
  • High-throughput screenings
  • Lipid bilayers

ASJC Scopus subject areas

  • Control and Systems Engineering

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