Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements

Takashi Ohtsuki, R. Vinayak, Y. I. Watanabe, K. Kita, G. Kawai, K. Watanabe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

RNA samples corresponding to Ascaris suum mitochondrial tRNA(Met) were chemically and automatically synthesized in amounts sufficient for NMR measurement. Conventional and rapid deprotection methods gave tRNA samples with the same amino acid-accepting activity as those prepared by other method; enzymatic synthesis, and enzymatic ligation of chemically synthesized fragments. The synthetic tRNA showed the same 1H-NMR spectrum in the iminoproton region as the ligated tRNA. This rapid and reliable preparation method thus provides biologically active tRNA for NMR measurement, and further, it is applicable for synthesis of other large synthetic RNAs, by combining the site-specific isotopic labeling method.

Original languageEnglish
Pages (from-to)1070-1073
Number of pages4
JournalJournal of Biochemistry
Volume120
Issue number6
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

RNA, Transfer, Met
Ascaris suum
Transfer RNA
Nuclear magnetic resonance
RNA
Labeling
Ligation
Binding Sites
Amino Acids

Keywords

  • Ascaris suum
  • Chemical synthesis
  • Mitochondria
  • NMR
  • tRNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNA(Met) toward NMR measurements. / Ohtsuki, Takashi; Vinayak, R.; Watanabe, Y. I.; Kita, K.; Kawai, G.; Watanabe, K.

In: Journal of Biochemistry, Vol. 120, No. 6, 1996, p. 1070-1073.

Research output: Contribution to journalArticle

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AU - Kita, K.

AU - Kawai, G.

AU - Watanabe, K.

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AB - RNA samples corresponding to Ascaris suum mitochondrial tRNA(Met) were chemically and automatically synthesized in amounts sufficient for NMR measurement. Conventional and rapid deprotection methods gave tRNA samples with the same amino acid-accepting activity as those prepared by other method; enzymatic synthesis, and enzymatic ligation of chemically synthesized fragments. The synthetic tRNA showed the same 1H-NMR spectrum in the iminoproton region as the ligated tRNA. This rapid and reliable preparation method thus provides biologically active tRNA for NMR measurement, and further, it is applicable for synthesis of other large synthetic RNAs, by combining the site-specific isotopic labeling method.

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