Automated chemical synthesis of biologically active tRNA having a sequence corresponding to Ascaris suum mitochondrial tRNAMet toward NMR measurements

Takashi Ohtsuki, Ravi Vinayak, Yoh Ichi Watanabe, Kiyoshi Kita, Gota Kawai, Kimitsuna Watanabe

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

RNA samples corresponding to Ascaris suum mitochondrial tRNAMet were chemically and automatically synthesized in amounts sufficient for NMR measurement. Conventional and rapid deprotection methods gave tRNA samples with the same amino acid-accepting activity as those prepared by other method; enzymatic synthesis, and enzymatic ligation of chemically synthesized fragments. The synthetic tRNA showed the same 1H-NMR spectrum in the iminoproton region as the ligated tRNA. This rapid and reliable preparation method thus provides biologically active tRNA for NMR measurement, and further, it is applicable for synthesis of other large synthetic RNAs, by combining the site-specific isotopic labeling method.

Original languageEnglish
Pages (from-to)1070-1073
Number of pages4
JournalJournal of biochemistry
Volume120
Issue number6
DOIs
Publication statusPublished - Dec 1996
Externally publishedYes

Keywords

  • Ascaris suum
  • Chemical synthesis
  • Mitochondria
  • NMR
  • tRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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