Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis

Jun Ichiro Tsuruha, Kayo Masuko-Hongo, Tomohiro Kato, Masahiro Sakata, Hiroshi Nakamura, Taichi Sekine, Masaharu Takigawa, Kusuki Nishioka

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Objective. Our previous study revealed that some patients with rheumatoid arthritis (RA) possessed autoantibodies to YKL-39, a cartilage related protein. We investigated whether patients with osteoarthritis (OA) also displayed autoimmunity to YKL-39. Methods. Autoantibodies to recombinant YKL-39 as well as human cartilage glycoprotein-39 were detected by ELISA and Western blotting. The tested serum samples were derived from 117 patients with OA, 94 patients with RA, and 2 groups of 50 arthropathy-free healthy donors who matched the OA and RA groups for age and sex. We determined autoepitopes on YKL-39 using 3 overlapping fragments of YKL-39 (designated F1, F2, F3). T cell proliferation response to YKL-39 was analyzed using the 3H-thymidine incorporation assay. Results. Autoantibodies to YKL-39 were detected in 13 (11.1%) patients with OA and 11 (11.8%) with RA. In the epitope mapping, all the 3 fragments of YKL-39 were found to carry autoepitopes, but F1 was recognized most frequently. Proliferative responses of peripheral blood mononuclear cells against YKL-39 were detected in 6 (46%) of the 13 OA patients who were positive for the anti-YKL-39 autoantibodies and in 2 (17%) of the 11 antibody positive RA patients. Conclusion. These results show that autoimmunity to YKL-39 in patients with OA was present at equal or somewhat higher frequency than in patients with RA. The cellular and humoral immune responses to YKL-39 may be involved in the pathological process of OA as well as RA.

Original languageEnglish
Pages (from-to)1459-1466
Number of pages8
JournalJournal of Rheumatology
Volume29
Issue number7
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Autoimmunity
Osteoarthritis
Cartilage
Rheumatoid Arthritis
Autoantibodies
Proteins
Epitope Mapping
Joint Diseases
Pathologic Processes
Humoral Immunity
Cellular Immunity
Thymidine
Blood Cells
Age Groups
Western Blotting
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Tissue Donors
T-Lymphocytes
Antibodies

Keywords

  • Autoantibody
  • Osteoarthritis
  • T cell immunity
  • YKL-39

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Tsuruha, J. I., Masuko-Hongo, K., Kato, T., Sakata, M., Nakamura, H., Sekine, T., ... Nishioka, K. (2002). Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. Journal of Rheumatology, 29(7), 1459-1466.

Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. / Tsuruha, Jun Ichiro; Masuko-Hongo, Kayo; Kato, Tomohiro; Sakata, Masahiro; Nakamura, Hiroshi; Sekine, Taichi; Takigawa, Masaharu; Nishioka, Kusuki.

In: Journal of Rheumatology, Vol. 29, No. 7, 2002, p. 1459-1466.

Research output: Contribution to journalArticle

Tsuruha, JI, Masuko-Hongo, K, Kato, T, Sakata, M, Nakamura, H, Sekine, T, Takigawa, M & Nishioka, K 2002, 'Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis', Journal of Rheumatology, vol. 29, no. 7, pp. 1459-1466.
Tsuruha JI, Masuko-Hongo K, Kato T, Sakata M, Nakamura H, Sekine T et al. Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. Journal of Rheumatology. 2002;29(7):1459-1466.
Tsuruha, Jun Ichiro ; Masuko-Hongo, Kayo ; Kato, Tomohiro ; Sakata, Masahiro ; Nakamura, Hiroshi ; Sekine, Taichi ; Takigawa, Masaharu ; Nishioka, Kusuki. / Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. In: Journal of Rheumatology. 2002 ; Vol. 29, No. 7. pp. 1459-1466.
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abstract = "Objective. Our previous study revealed that some patients with rheumatoid arthritis (RA) possessed autoantibodies to YKL-39, a cartilage related protein. We investigated whether patients with osteoarthritis (OA) also displayed autoimmunity to YKL-39. Methods. Autoantibodies to recombinant YKL-39 as well as human cartilage glycoprotein-39 were detected by ELISA and Western blotting. The tested serum samples were derived from 117 patients with OA, 94 patients with RA, and 2 groups of 50 arthropathy-free healthy donors who matched the OA and RA groups for age and sex. We determined autoepitopes on YKL-39 using 3 overlapping fragments of YKL-39 (designated F1, F2, F3). T cell proliferation response to YKL-39 was analyzed using the 3H-thymidine incorporation assay. Results. Autoantibodies to YKL-39 were detected in 13 (11.1{\%}) patients with OA and 11 (11.8{\%}) with RA. In the epitope mapping, all the 3 fragments of YKL-39 were found to carry autoepitopes, but F1 was recognized most frequently. Proliferative responses of peripheral blood mononuclear cells against YKL-39 were detected in 6 (46{\%}) of the 13 OA patients who were positive for the anti-YKL-39 autoantibodies and in 2 (17{\%}) of the 11 antibody positive RA patients. Conclusion. These results show that autoimmunity to YKL-39 in patients with OA was present at equal or somewhat higher frequency than in patients with RA. The cellular and humoral immune responses to YKL-39 may be involved in the pathological process of OA as well as RA.",
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AU - Masuko-Hongo, Kayo

AU - Kato, Tomohiro

AU - Sakata, Masahiro

AU - Nakamura, Hiroshi

AU - Sekine, Taichi

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N2 - Objective. Our previous study revealed that some patients with rheumatoid arthritis (RA) possessed autoantibodies to YKL-39, a cartilage related protein. We investigated whether patients with osteoarthritis (OA) also displayed autoimmunity to YKL-39. Methods. Autoantibodies to recombinant YKL-39 as well as human cartilage glycoprotein-39 were detected by ELISA and Western blotting. The tested serum samples were derived from 117 patients with OA, 94 patients with RA, and 2 groups of 50 arthropathy-free healthy donors who matched the OA and RA groups for age and sex. We determined autoepitopes on YKL-39 using 3 overlapping fragments of YKL-39 (designated F1, F2, F3). T cell proliferation response to YKL-39 was analyzed using the 3H-thymidine incorporation assay. Results. Autoantibodies to YKL-39 were detected in 13 (11.1%) patients with OA and 11 (11.8%) with RA. In the epitope mapping, all the 3 fragments of YKL-39 were found to carry autoepitopes, but F1 was recognized most frequently. Proliferative responses of peripheral blood mononuclear cells against YKL-39 were detected in 6 (46%) of the 13 OA patients who were positive for the anti-YKL-39 autoantibodies and in 2 (17%) of the 11 antibody positive RA patients. Conclusion. These results show that autoimmunity to YKL-39 in patients with OA was present at equal or somewhat higher frequency than in patients with RA. The cellular and humoral immune responses to YKL-39 may be involved in the pathological process of OA as well as RA.

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