Aurora kinase-A overexpression in mouse mammary epithelium induces mammary adenocarcinomas harboring genetic alterations shared with human breast cancer

Warapen Treekitkarnmongkol, Hiroshi Katayama, Kazuharu Kai, Kaori Sasai, Jennifer Carter Jones, Jing Wang, Li Shen, Aysegul A. Sahin, Mihai Gagea, Naoto T. Ueno, Chad J. Creighton, Subrata Sen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recent data from The Cancer Genome Atlas analysis have revealed that Aurora kinase A (AURKA) amplification and overexpression characterize a distinct subset of human tumors across multiple cancer types. Although elevated expression of AURKA has been shown to induce oncogenic phenotypes in cells in vitro, findings from transgenic mouse models of Aurora-A overexpression in mammary glands have been distinct depending on the models generated. In the present study, we report that prolonged overexpression of AURKA transgene in mammary epithelium driven by ovine β-lactoglobulin promoter, activated through multiple pregnancy and lactation cycles, results in the development of mammary adenocarcinomas with alterations in cancer-relevant genes and epithelial-to-mesenchymal transition. The tumor incidence was 38.9% (7/18) in Aurora-A transgenic mice at 16 months of age following 4-5 pregnancy cycles. Aurora-A overexpression in the tumor tissues accompanied activation of Akt, elevation of Cyclin D1, Tpx2 and Plk1 along with downregulation of ERa and p53 proteins, albeit at varying levels. Microarray comparative genomic hybridization (CGH) analyses of transgenic mouse mammary adenocarcinomas revealed copy gain of Glp1r and losses of Ercc5, Pten and Tcf7l2 loci. Review of human breast tumor transcriptomic data sets showed association of these genes at varying levels with Aurora-A gain of function alterations. Whole exome sequencing of the mouse tumors also identified gene mutations detected in Aurora-A overexpressing human breast cancers. Our findings demonstrate that prolonged overexpression of Aurora-A can be a driver somatic genetic event in mammary adenocarcinomas associated with deregulated tumor-relevant pathways in the Aurora-A subset of human breast cancer.

Original languageEnglish
Pages (from-to)1180-1189
Number of pages10
JournalCarcinogenesis
Volume37
Issue number12
DOIs
Publication statusPublished - 2016

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Aurora Kinase A
Adenocarcinoma
Breast
Epithelium
Breast Neoplasms
Neoplasms
Transgenic Mice
Exome
Lactoglobulins
Multiple Pregnancy
Epithelial-Mesenchymal Transition
Comparative Genomic Hybridization
Atlases
Neoplasm Genes
Cyclin D1
Human Mammary Glands
Transgenes
Lactation
Genes
Sheep

ASJC Scopus subject areas

  • Cancer Research

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Aurora kinase-A overexpression in mouse mammary epithelium induces mammary adenocarcinomas harboring genetic alterations shared with human breast cancer. / Treekitkarnmongkol, Warapen; Katayama, Hiroshi; Kai, Kazuharu; Sasai, Kaori; Jones, Jennifer Carter; Wang, Jing; Shen, Li; Sahin, Aysegul A.; Gagea, Mihai; Ueno, Naoto T.; Creighton, Chad J.; Sen, Subrata.

In: Carcinogenesis, Vol. 37, No. 12, 2016, p. 1180-1189.

Research output: Contribution to journalArticle

Treekitkarnmongkol, W, Katayama, H, Kai, K, Sasai, K, Jones, JC, Wang, J, Shen, L, Sahin, AA, Gagea, M, Ueno, NT, Creighton, CJ & Sen, S 2016, 'Aurora kinase-A overexpression in mouse mammary epithelium induces mammary adenocarcinomas harboring genetic alterations shared with human breast cancer', Carcinogenesis, vol. 37, no. 12, pp. 1180-1189. https://doi.org/10.1093/carcin/bgw097
Treekitkarnmongkol, Warapen ; Katayama, Hiroshi ; Kai, Kazuharu ; Sasai, Kaori ; Jones, Jennifer Carter ; Wang, Jing ; Shen, Li ; Sahin, Aysegul A. ; Gagea, Mihai ; Ueno, Naoto T. ; Creighton, Chad J. ; Sen, Subrata. / Aurora kinase-A overexpression in mouse mammary epithelium induces mammary adenocarcinomas harboring genetic alterations shared with human breast cancer. In: Carcinogenesis. 2016 ; Vol. 37, No. 12. pp. 1180-1189.
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