Augmentation by 2-Mercaptoethanol of In Vitro Anti-TNP Antibody Production Induced by Butyrate Plus IL-2 in Murine Splenic B Cells

Eiichi Gohda, Takayuki Okamura, Eriko Aoyama, Itaru Yamamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.

Original languageEnglish
Pages (from-to)539-550
Number of pages12
JournalImmunopharmacology and Immunotoxicology
Volume25
Issue number4
DOIs
Publication statusPublished - 2003

Fingerprint

Mercaptoethanol
Butyrates
Antibody Formation
Interleukin-2
Anti-Idiotypic Antibodies
B-Lymphocytes
Cells
Antibodies
Cysteamine
Butyric Acid
Volatile Fatty Acids
Dithiothreitol
Sulfhydryl Compounds
Glutathione
Immunoglobulin M
In Vitro Techniques
Assays
Carbon
Enzyme-Linked Immunosorbent Assay
Atoms

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Augmentation by 2-Mercaptoethanol of In Vitro Anti-TNP Antibody Production Induced by Butyrate Plus IL-2 in Murine Splenic B Cells. / Gohda, Eiichi; Okamura, Takayuki; Aoyama, Eriko; Yamamoto, Itaru.

In: Immunopharmacology and Immunotoxicology, Vol. 25, No. 4, 2003, p. 539-550.

Research output: Contribution to journalArticle

@article{5e8c295d591a4fcfae7e65f6845343ee,
title = "Augmentation by 2-Mercaptoethanol of In Vitro Anti-TNP Antibody Production Induced by Butyrate Plus IL-2 in Murine Splenic B Cells",
abstract = "We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.",
author = "Eiichi Gohda and Takayuki Okamura and Eriko Aoyama and Itaru Yamamoto",
year = "2003",
doi = "10.1081/IPH-120026439",
language = "English",
volume = "25",
pages = "539--550",
journal = "Immunopharmacology and Immunotoxicology",
issn = "0892-3973",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Augmentation by 2-Mercaptoethanol of In Vitro Anti-TNP Antibody Production Induced by Butyrate Plus IL-2 in Murine Splenic B Cells

AU - Gohda, Eiichi

AU - Okamura, Takayuki

AU - Aoyama, Eriko

AU - Yamamoto, Itaru

PY - 2003

Y1 - 2003

N2 - We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.

AB - We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.

UR - http://www.scopus.com/inward/record.url?scp=0345167013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345167013&partnerID=8YFLogxK

U2 - 10.1081/IPH-120026439

DO - 10.1081/IPH-120026439

M3 - Article

C2 - 14686796

AN - SCOPUS:0345167013

VL - 25

SP - 539

EP - 550

JO - Immunopharmacology and Immunotoxicology

JF - Immunopharmacology and Immunotoxicology

SN - 0892-3973

IS - 4

ER -