Augmentation by 2-Mercaptoethanol of In Vitro Anti-TNP Antibody Production Induced by Butyrate Plus IL-2 in Murine Splenic B Cells

Eiichi Gohda, Takayuki Okamura, Eriko Aoyama, Itaru Yamamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.

Original languageEnglish
Pages (from-to)539-550
Number of pages12
JournalImmunopharmacology and Immunotoxicology
Volume25
Issue number4
DOIs
Publication statusPublished - Dec 19 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

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