Attenuation of oxidative DNA damage with a novel antioxidant EPC-K1 in rat brain neuronal cells after transient middle cerebral artery occlusion

W. R. Zhang, T. Hayashi, C. Sasaki, K. Sato, I. Nagano, Y. Manabe, Koji Abe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

EPC-K1, L-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1- benzopyran-6-yl-hydrogen phosphate] potassium salt, is a novel antioxidant. In this study, we investigated a reduction of oxidative neuronal cell damage with EPC-K1 by immunohistochemical analysis for 8-hydroxy-2′-deoxyguanosine (8-OHdG) in rat brain with 60 min transient middle cerebral artery occlusion, in association with terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNED and staining for total and active caspase-3. Treatment with EPC-K1 (20 mg kg-1 i.v.) significantly reduced infarct size (p <0.05) at 24 h of reperfusion. There were no positive cells for 8-OHdG and TUNEL in sham-operated brain, but numerous cells became positive for 8-OHdG, TUNEL and caspase-3 in the brains with ischemia. The number was markedly reduced in the EPC-K1 treated group. These reductions were particularly evident in the border zone of the infarct area, but the degree of reduction was less in caspase-3 staining than in 8-OHdG and TUNEL stainings. These results indicate EPC-K1 attenuates oxidative neuronal cell damage and prevents neuronal cell death.

Original languageEnglish
Pages (from-to)676-680
Number of pages5
JournalNeurological Research
Volume23
Issue number6
DOIs
Publication statusPublished - 2001

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Keywords

  • 8-OHdG
  • Caspase-3
  • EPC-K1
  • Ischemia
  • TUNEL

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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