Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG

Yuki Arimura, Yusuke Minato, Takayuki Wada, Masaaki Nakayama, Ayako Ryumon, Nao Hirata, Chie Nakajima, Yasuhiko Suzuki, Manabu Ato, Kazuo Kobayashi, Naoko Ohara, Seiji Iida, Naoya Ohara

Research output: Contribution to journalArticlepeer-review

Abstract

Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.

Original languageEnglish
JournalMICROBIOLOGY and IMMUNOLOGY
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • BCG
  • Mycobacterium
  • thymidylate synthase
  • ThyX

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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