AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana

Takashi Hirayama, Norihiro Mitsukawa, Daisuke Shibata, Kazuo Shinozaki

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) from the higher plant Arabidopsis thaliana was cloned and characterized. The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predicted AtPLC2 protein is similar to those of plant PI-PLCs and mammalian δ-type PI-PLCs. Northern blot analysis revealed that AtPLC2 is expressed constitutively whereas AtPLC1S, another gene for PI-PLC of Arabidopsis, is induced by environmental stresses such as dehydration and salinity, indicating that the function of AtPLC2 is distinct from that of AtPLC1S. The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalPlant Molecular Biology
Volume34
Issue number1
DOIs
Publication statusPublished - May 1997
Externally publishedYes

Fingerprint

Phosphoinositide Phospholipase C
Gene encoding
phospholipase C
Type C Phospholipases
Programmable logic controllers
Phosphatidylinositols
Arabidopsis
Arabidopsis thaliana
Genes
Tissue
Complementary DNA
genes
Chromosome Mapping
Salinity
Chromosomes
Dehydration
Northern blotting
Restriction Fragment Length Polymorphisms
Northern Blotting
restriction fragment length polymorphism

Keywords

  • Arabidopsis thaliana
  • chromosomal mapping
  • phosphoinositide-specific phospholipase C

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana. / Hirayama, Takashi; Mitsukawa, Norihiro; Shibata, Daisuke; Shinozaki, Kazuo.

In: Plant Molecular Biology, Vol. 34, No. 1, 05.1997, p. 175-180.

Research output: Contribution to journalArticle

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