AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana

Takashi Hirayama, Norihiro Mitsukawa, Daisuke Shibata, Kazuo Shinozaki

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) from the higher plant Arabidopsis thaliana was cloned and characterized. The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predicted AtPLC2 protein is similar to those of plant PI-PLCs and mammalian δ-type PI-PLCs. Northern blot analysis revealed that AtPLC2 is expressed constitutively whereas AtPLC1S, another gene for PI-PLC of Arabidopsis, is induced by environmental stresses such as dehydration and salinity, indicating that the function of AtPLC2 is distinct from that of AtPLC1S. The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalPlant Molecular Biology
Volume34
Issue number1
DOIs
Publication statusPublished - May 1 1997
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • chromosomal mapping
  • phosphoinositide-specific phospholipase C

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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