ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana

Daisuke Saisho, Mikio Nakazono, Nobuhiro Tsutsumi, Atsushi Hirai

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOXla transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NAN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalJournal of Plant Physiology
Volume158
Issue number2
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Arabidopsis
Arabidopsis thaliana
Adenosine Triphosphate
Messenger RNA
Mitochondria
mitochondria
synthesis
oligomycin
Genes
Antimycin A
antimycin A
2,4-Dinitrophenol
Oligomycins
H-transporting ATP synthase
Electron Transport Complex III
gene induction
electron transport chain
Cytochromes
cytochromes
Electron Transport

Keywords

  • Alternative oxidase
  • Arabidopsis thaliana
  • ATP synthesis inhibitor
  • Gene expression
  • Respiratory inhibitor

ASJC Scopus subject areas

  • Plant Science

Cite this

ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana. / Saisho, Daisuke; Nakazono, Mikio; Tsutsumi, Nobuhiro; Hirai, Atsushi.

In: Journal of Plant Physiology, Vol. 158, No. 2, 2001, p. 241-245.

Research output: Contribution to journalArticle

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