Abstract
The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOXla transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NAN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.
| Original language | English |
|---|---|
| Pages (from-to) | 241-245 |
| Number of pages | 5 |
| Journal | Journal of Plant Physiology |
| Volume | 158 |
| Issue number | 2 |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Alternative oxidase
- Arabidopsis thaliana
- ATP synthesis inhibitor
- Gene expression
- Respiratory inhibitor
ASJC Scopus subject areas
- Plant Science
Cite this
ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana. / Saisho, Daisuke; Nakazono, Mikio; Tsutsumi, Nobuhiro; Hirai, Atsushi.
In: Journal of Plant Physiology, Vol. 158, No. 2, 2001, p. 241-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana
AU - Saisho, Daisuke
AU - Nakazono, Mikio
AU - Tsutsumi, Nobuhiro
AU - Hirai, Atsushi
PY - 2001
Y1 - 2001
N2 - The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOXla transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NAN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.
AB - The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOXla transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NAN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.
KW - Alternative oxidase
KW - Arabidopsis thaliana
KW - ATP synthesis inhibitor
KW - Gene expression
KW - Respiratory inhibitor
UR - http://www.scopus.com/inward/record.url?scp=0035138473&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035138473&partnerID=8YFLogxK
M3 - Article
VL - 158
SP - 241
EP - 245
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 2
ER -