TY - JOUR
T1 - ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase mRNA in Arabidopsis thaliana
AU - Saisho, Daisuke
AU - Nakazono, Mikio
AU - Tsutsumi, Nobuhiro
AU - Hirai, Atsushi
N1 - Funding Information:
Acknowledgements. We would like to thank Drs. N. Minagawa, S. Sakajo, S. Akita, H. Yukioka and K.H. Lee for helpful discussions. This work was supported by grants from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and by grants-in-aid from the Ministry of Education, Science and Culture of Japan.
PY - 2001
Y1 - 2001
N2 - The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOXla transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NAN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.
AB - The oxygen uptake in mitochondria can be uncoupled from ATP synthesis by the product of a single gene, the alternative oxidase (AOX) gene, which may be involved in signal transduction from mitochondria to the nucleus. To better understand induction of this gene in higher plants, we investigated the effects of the cytochrome respiratory inhibitors and ATP synthesis inhibitors on the expression of the AOXs of Arabidopsis thaliana. A Northern blot analysis showed the AOXla transcripts were increased by treatment of antimycin A and myxothiazol, which act on complex III of the respiratory chain, and by NAN3, which acts on complex IV. AOX1a mRNA was also strongly induced by oligomycin (F1F0-ATP synthase inhibitor) and weakly induced by 2,4-DNP (ATP synthesis uncoupler) at the maximum dose. These results indicate that not only inhibition of electron transfer but also inhibition of ATP synthesis in mitochondria induces the transcription of AOX1a.
KW - ATP synthesis inhibitor
KW - Alternative oxidase
KW - Arabidopsis thaliana
KW - Gene expression
KW - Respiratory inhibitor
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U2 - 10.1078/0176-1617-00193
DO - 10.1078/0176-1617-00193
M3 - Article
AN - SCOPUS:0035138473
VL - 158
SP - 241
EP - 245
JO - Z. PFLANZENPHYSIOL.
JF - Z. PFLANZENPHYSIOL.
SN - 0176-1617
IS - 2
ER -