Abstract
Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organelles using chromaffin granule vesicles and proteoliposomes reconstituted with purified F-type H+-ATPase as model systems. Radiolabeled daunomycin was taken up by chromaffin vesicles upon addition of ATP. Its ATP-dependent uptake was stimulated about 1.4- to 1.8-fold by valinomycin plus K+, but was inhibited by ammonium chloride (10 mM) and nigericin plus K+. Quinidine (5μM), verapamil (5 μM), or vanadate (0.5 mM), inhibitors of P-glycoprotein, had no effect on its uptake. Daunomycin was also taken up by liposomes reconstituted with F-type H+-ATPase. Furthermore, doxorubicin and vinblastine were taken up by these vesicles, whereas colchicine and rhodamine 123 were not. The accumulations of daunomycin and doxorubicin in acidic organelles of cultured cells were decreased by inhibiting vacuolar ATPase by addition of bafilomycin A, or concanamycin A, or by increasing the internal ΔpH by addition of nigericin. Melittin and N-(6-aminohexyl)-5-chloro-l-naphthalenesulfonamide dissipated the dpH and inhibited accumulation of daunomycin in the membrane vesicles and acidic organelles in cultured cells. These results indicate that the ΔpH established by vacuolartype ATPase drives the uptake of daunomycin, doxorubicin or vinblastine into acidic organelles, and that no specific transporters are involved in their uptakes.
| Original language | English |
|---|---|
| Pages (from-to) | 213-218 |
| Number of pages | 6 |
| Journal | Journal of Biochemistry |
| Volume | 115 |
| Issue number | 2 |
| Publication status | Published - Feb 1994 |
| Externally published | Yes |
Fingerprint
Keywords
- Acidic organelles
- Daunomycin
- Multidrug resistance
- P-glycoprotein
- Vacuolar ATPase
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Molecular Biology
- Biochemistry
Cite this
ATP-dependent uptake of anti-neoplastic agents by acidic organelles. / Moriyama, Yoshinori; Manabe, Tsukasa; Yoshimori, Tamotsu; Tashiro, Yutaka; Futai, Masamitsu.
In: Journal of Biochemistry, Vol. 115, No. 2, 02.1994, p. 213-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ATP-dependent uptake of anti-neoplastic agents by acidic organelles
AU - Moriyama, Yoshinori
AU - Manabe, Tsukasa
AU - Yoshimori, Tamotsu
AU - Tashiro, Yutaka
AU - Futai, Masamitsu
PY - 1994/2
Y1 - 1994/2
N2 - Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organelles using chromaffin granule vesicles and proteoliposomes reconstituted with purified F-type H+-ATPase as model systems. Radiolabeled daunomycin was taken up by chromaffin vesicles upon addition of ATP. Its ATP-dependent uptake was stimulated about 1.4- to 1.8-fold by valinomycin plus K+, but was inhibited by ammonium chloride (10 mM) and nigericin plus K+. Quinidine (5μM), verapamil (5 μM), or vanadate (0.5 mM), inhibitors of P-glycoprotein, had no effect on its uptake. Daunomycin was also taken up by liposomes reconstituted with F-type H+-ATPase. Furthermore, doxorubicin and vinblastine were taken up by these vesicles, whereas colchicine and rhodamine 123 were not. The accumulations of daunomycin and doxorubicin in acidic organelles of cultured cells were decreased by inhibiting vacuolar ATPase by addition of bafilomycin A, or concanamycin A, or by increasing the internal ΔpH by addition of nigericin. Melittin and N-(6-aminohexyl)-5-chloro-l-naphthalenesulfonamide dissipated the dpH and inhibited accumulation of daunomycin in the membrane vesicles and acidic organelles in cultured cells. These results indicate that the ΔpH established by vacuolartype ATPase drives the uptake of daunomycin, doxorubicin or vinblastine into acidic organelles, and that no specific transporters are involved in their uptakes.
AB - Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organelles using chromaffin granule vesicles and proteoliposomes reconstituted with purified F-type H+-ATPase as model systems. Radiolabeled daunomycin was taken up by chromaffin vesicles upon addition of ATP. Its ATP-dependent uptake was stimulated about 1.4- to 1.8-fold by valinomycin plus K+, but was inhibited by ammonium chloride (10 mM) and nigericin plus K+. Quinidine (5μM), verapamil (5 μM), or vanadate (0.5 mM), inhibitors of P-glycoprotein, had no effect on its uptake. Daunomycin was also taken up by liposomes reconstituted with F-type H+-ATPase. Furthermore, doxorubicin and vinblastine were taken up by these vesicles, whereas colchicine and rhodamine 123 were not. The accumulations of daunomycin and doxorubicin in acidic organelles of cultured cells were decreased by inhibiting vacuolar ATPase by addition of bafilomycin A, or concanamycin A, or by increasing the internal ΔpH by addition of nigericin. Melittin and N-(6-aminohexyl)-5-chloro-l-naphthalenesulfonamide dissipated the dpH and inhibited accumulation of daunomycin in the membrane vesicles and acidic organelles in cultured cells. These results indicate that the ΔpH established by vacuolartype ATPase drives the uptake of daunomycin, doxorubicin or vinblastine into acidic organelles, and that no specific transporters are involved in their uptakes.
KW - Acidic organelles
KW - Daunomycin
KW - Multidrug resistance
KW - P-glycoprotein
KW - Vacuolar ATPase
UR - http://www.scopus.com/inward/record.url?scp=0028052884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028052884&partnerID=8YFLogxK
M3 - Article
VL - 115
SP - 213
EP - 218
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 2
ER -