ATP-citrate lyase from the green sulfur bacterium Chlorobium limicola is a heteromeric enzyme composed of two distinct gene products

Tadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi, Tadayuki Imanaka

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The reductive tricarboxylic acid cycle functions as a carbon dioxide fixation pathway in the green sulfur bacterium, Chlorobium limicola. ATP-citrate lyase, one of the key enzymes of this cycle, was partially purified from C. limicola strain M1 and the N-terminal sequence of a 65-kDa protein was found to show similarity toward eukaryotic ATP-citrate lyase. A DNA fragment was amplified with primers designed from this sequence and an internal sequence highly conserved among eukaryotic enzymes. Using this fragment as a probe, we isolated a DNA fragment containing two adjacent open reading frames, aclB (1197 bp) and aclA (1827 bp), whose products showed significant similarity to the N- and C-terminal regions of the human enzyme, respectively. Heterologous expression of these genes in Escherichia coli showed that both gene products were essential for ATP-citrate lyase activity. The recombinant enzyme was purified from the cell-free extract of E. coli harboring aclBA for further characterization. The molecular mass of the recombinant enzyme was determined to be approximately 532-557 kDa by gel-filtration. The enzyme catalyzed the cleavage of citrate in an ATP-, CoA- and Mg2+-dependent manner, where ATP and Mg2+ could be replaced by dATP and Mn2+, respectively. ADP and oxaloacetate inhibited the reaction. These properties suggested that ATP-citrate lyase from C. limicola controlled the cycle flux depending on intracellular energy conditions. This paper provides the first direct evidence that a bacterial ATP-citrate lyase is a heteromeric enzyme, distinct from mammalian enzymes.

Original languageEnglish
Pages (from-to)1670-1678
Number of pages9
JournalEuropean Journal of Biochemistry
Volume268
Issue number6
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Chlorobium
Chlorobi
ATP Citrate (pro-S)-Lyase
Sulfur
Bacteria
Genes
Enzymes
Escherichia coli
Adenosine Triphosphate
Nitrogen fixation
Carbon Cycle
Oxaloacetic Acid
Citric Acid Cycle
Conserved Sequence
DNA
Molecular mass
Coenzyme A
Cell Extracts
Carbon Dioxide
Adenosine Diphosphate

Keywords

  • ATP-citrate lyase
  • Chlorobium limicola
  • Reductive tricarboxylic acid cycle

ASJC Scopus subject areas

  • Biochemistry

Cite this

ATP-citrate lyase from the green sulfur bacterium Chlorobium limicola is a heteromeric enzyme composed of two distinct gene products. / Kanao, Tadayoshi; Fukui, Toshiaki; Atomi, Haruyuki; Imanaka, Tadayuki.

In: European Journal of Biochemistry, Vol. 268, No. 6, 2001, p. 1670-1678.

Research output: Contribution to journalArticle

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