ATML1 and PDF2 play a redundant and essential role in arabidopsis embryo development

Eriko Ogawa, Yusuke Yamada, Noriko Sezaki, Sho Kosaka, Hitoshi Kondo, Naoko Kamata, Mitsutomo Abe, Yoshibumi Komeda, Taku Takahashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The epidermis of shoot organs in plants develops from the outermost layer (L1) of the shoot apical meristem. In Arabidopsis, a pair of homeobox genes, ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1) and PROTODERMAL FACTOR2 (PDF2), play a role in regulating the expression of L1-specific genes. atml1-1 pdf2-1 double mutants show striking defects in the differentiation of shoot epidermal cells. However, because atml1-1 and pdf2-1 have a T-DNA inserted downstream of the respective homeobox sequences, these alleles may not represent null mutations. Here we characterized additional mutant alleles that have a T-DNA insertion at different positions of each gene. Double mutants of a strong atml1-3 allele with each pdf2 allele were found to cause embryonic arrest at the globular stage. Although with low frequency, all double mutant combinations of a weak atml1-1 allele with each pdf2 allele germinated and showed phenotypes defective in shoot epidermal cell differentiation. We further confirmed that transgenic induction of PDF2 fused to the Drosophila Engrailed repressor domain temporarily interferes with epidermal cell differentiation in the wild-type background. These results indicate that ATML1 and PDF2 act redundantly as a positive regulator of shoot epidermal cell differentiation and at least one copy of these genes is essential for embryo development.

Original languageEnglish
Pages (from-to)1183-1192
Number of pages10
JournalPlant and Cell Physiology
Volume56
Issue number6
DOIs
Publication statusPublished - Dec 22 2014

Fingerprint

Meristem
Arabidopsis
meristems
Embryonic Development
embryogenesis
Arabidopsis thaliana
Alleles
alleles
cell differentiation
Cell Differentiation
mutants
shoots
Homeobox Genes
homeotic genes
Gene Order
genes
shoot meristems
Essential Genes
DNA
apical meristems

Keywords

  • Arabidopsis thaliana
  • ATML1
  • Embryo
  • Epidermis
  • HD-Zip IV
  • PDF2

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

ATML1 and PDF2 play a redundant and essential role in arabidopsis embryo development. / Ogawa, Eriko; Yamada, Yusuke; Sezaki, Noriko; Kosaka, Sho; Kondo, Hitoshi; Kamata, Naoko; Abe, Mitsutomo; Komeda, Yoshibumi; Takahashi, Taku.

In: Plant and Cell Physiology, Vol. 56, No. 6, 22.12.2014, p. 1183-1192.

Research output: Contribution to journalArticle

Ogawa, E, Yamada, Y, Sezaki, N, Kosaka, S, Kondo, H, Kamata, N, Abe, M, Komeda, Y & Takahashi, T 2014, 'ATML1 and PDF2 play a redundant and essential role in arabidopsis embryo development', Plant and Cell Physiology, vol. 56, no. 6, pp. 1183-1192. https://doi.org/10.1093/pcp/pcv045
Ogawa E, Yamada Y, Sezaki N, Kosaka S, Kondo H, Kamata N et al. ATML1 and PDF2 play a redundant and essential role in arabidopsis embryo development. Plant and Cell Physiology. 2014 Dec 22;56(6):1183-1192. https://doi.org/10.1093/pcp/pcv045
Ogawa, Eriko ; Yamada, Yusuke ; Sezaki, Noriko ; Kosaka, Sho ; Kondo, Hitoshi ; Kamata, Naoko ; Abe, Mitsutomo ; Komeda, Yoshibumi ; Takahashi, Taku. / ATML1 and PDF2 play a redundant and essential role in arabidopsis embryo development. In: Plant and Cell Physiology. 2014 ; Vol. 56, No. 6. pp. 1183-1192.
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