AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis

Kentaro Takei, Nanae Ueda, Koh Aoki, Takashi Kuromori, Takashi Hirayama, Kazuo Shinozaki, Tomoyuki Yamaya, Hitoshi Sakakibara

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

We analyzed the spatial expression pattern of Arabidopsis thaliana adenosine phosphates-isopentenyltransferase genes (AtIPT1, AtIPT3 to AtIPT8) and the effect of inorganic nitrogen sources on their regulation. In mature plants, the AtIPTs were differentially expressed in various tissues including the roots, leaves, stems, flowers and siliques. In transgenic seedlings expressing a gene for green fluorescent protein (GFP) driven by the AtIPT promoters, AtIPT1::GFP was predominantly expressed in the vascular stele of the roots, AtIPT3::GFP was in the phloem companion cells, AtIPT5::GFP was in the lateral root primordium and pericycle, and AtIPT7::GFP was in both the vascular stele and the phloem companion cells of the roots. In a long-term treatment, the accumulation level of AtIPT5 transcript was correlated with the concentrations of NO3- and NH4+ in the growth medium. However, under nitrogen-limited conditions, AtIPT3 expression was rapidly induced by NO3- in the seedlings accompanying the accumulation of cytokinins, whereas AtIPT5 expression was little affected. The NO3--dependent accumulation of both the AtIPT3 transcript and the cytokinins was markedly reduced in a Ds transposon-insertion mutant of AtIPT3. These results suggest that nitrogen availability differentially regulates expression of AtIPT3 and AtIPT5, and that AtIPT3 is a key determinant of cytokinin biosynthesis in response to rapid changes in the availability of NO3-.

Original languageEnglish
Pages (from-to)1053-1062
Number of pages10
JournalPlant and Cell Physiology
Volume45
Issue number8
Publication statusPublished - Aug 2004
Externally publishedYes

Fingerprint

Cytokinins
Green Fluorescent Proteins
green fluorescent protein
Arabidopsis
Nitrates
cytokinins
nitrates
biosynthesis
phloem companion cells
Phloem
stele
Nitrogen
Seedlings
blood vessels
Blood Vessels
nitrogen
root primordia
Adenine Nucleotides
seedlings
adenosine

Keywords

  • Ammonium ions
  • Arabidopsis
  • Cytokinin
  • Isopentenyltransferase
  • Nitrate ions

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Takei, K., Ueda, N., Aoki, K., Kuromori, T., Hirayama, T., Shinozaki, K., ... Sakakibara, H. (2004). AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis. Plant and Cell Physiology, 45(8), 1053-1062.

AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis. / Takei, Kentaro; Ueda, Nanae; Aoki, Koh; Kuromori, Takashi; Hirayama, Takashi; Shinozaki, Kazuo; Yamaya, Tomoyuki; Sakakibara, Hitoshi.

In: Plant and Cell Physiology, Vol. 45, No. 8, 08.2004, p. 1053-1062.

Research output: Contribution to journalArticle

Takei, K, Ueda, N, Aoki, K, Kuromori, T, Hirayama, T, Shinozaki, K, Yamaya, T & Sakakibara, H 2004, 'AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis', Plant and Cell Physiology, vol. 45, no. 8, pp. 1053-1062.
Takei K, Ueda N, Aoki K, Kuromori T, Hirayama T, Shinozaki K et al. AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis. Plant and Cell Physiology. 2004 Aug;45(8):1053-1062.
Takei, Kentaro ; Ueda, Nanae ; Aoki, Koh ; Kuromori, Takashi ; Hirayama, Takashi ; Shinozaki, Kazuo ; Yamaya, Tomoyuki ; Sakakibara, Hitoshi. / AtIPT3 is a key determinant of nitrate-dependent cytokinin biosynthesis in Arabidopsis. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 8. pp. 1053-1062.
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