Asymmetric segregation of the homeodomain protein Prospero duringDrosophila development

Joe Hirata, Hideki Nakagoshi, Yo Ichi Nabeshima, Fumio Matsuzaki

Research output: Contribution to journalArticle

281 Citations (Scopus)

Abstract

ASYMMETRIC divisions that produce two distinct cells play fundamental roles in generating different cell types during development1,2. In the Drosophilacentral nervous system, neural stem cells called neuroblasts divide unequally into another neuro-blast and a ganglion mother cell which is subsequently cleaved into neurons. Correct gene expression of ganglion mother cells requires the transcription factor Prospero3-5. Here we demonstrate the asymmetric segregation of Prospero on neuroblast division. Prospero synthesized in neuroblasts is retained in the cytoplasm and at mitosis is exclusively partitioned to ganglion mother cells, in which it is translocated to the nucleus. Differential segregation of Prospero was also found in the endoderm. We have identified a region in Prospero that is responsible for this event. The region shares a common motif with Numb6, which also shows unequal segregation7. We propose that asymmetric segregation of transcription factors is an intrinsic mechanism for establishing asymmetry in gene expression between sibling cells.

Original languageEnglish
Pages (from-to)627-630
Number of pages4
JournalNature
Volume377
Issue number6550
Publication statusPublished - 1995
Externally publishedYes

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Homeodomain Proteins
Ganglia
Stem Cells
Transcription Factors
Gene Expression
Endoderm
Neural Stem Cells
Mitosis
Nervous System
Cytoplasm
Neurons

ASJC Scopus subject areas

  • General

Cite this

Hirata, J., Nakagoshi, H., Nabeshima, Y. I., & Matsuzaki, F. (1995). Asymmetric segregation of the homeodomain protein Prospero duringDrosophila development. Nature, 377(6550), 627-630.

Asymmetric segregation of the homeodomain protein Prospero duringDrosophila development. / Hirata, Joe; Nakagoshi, Hideki; Nabeshima, Yo Ichi; Matsuzaki, Fumio.

In: Nature, Vol. 377, No. 6550, 1995, p. 627-630.

Research output: Contribution to journalArticle

Hirata, J, Nakagoshi, H, Nabeshima, YI & Matsuzaki, F 1995, 'Asymmetric segregation of the homeodomain protein Prospero duringDrosophila development', Nature, vol. 377, no. 6550, pp. 627-630.
Hirata, Joe ; Nakagoshi, Hideki ; Nabeshima, Yo Ichi ; Matsuzaki, Fumio. / Asymmetric segregation of the homeodomain protein Prospero duringDrosophila development. In: Nature. 1995 ; Vol. 377, No. 6550. pp. 627-630.
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