Asymmetric reduction of a variety of ketones with a recombinant carbonyl reductase: Identification of the gene encoding a versatile biocatalyst

Tadashi Ema, Hideo Yagasaki, Nobuyasu Okita, Kumiko Nishikawa, Toshinobu Korenaga, Takashi Sakai

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The gene encoding a versatile biocatalyst that shows high enantioselectivity for a variety of ketones, SCR (Saccharomyces cerevisiae carbonyl reductase), has been identified, cloned, and expressed in E. coli. Recombinant E. coli co-producing SCR and GDH (glucose dehydrogenase) is an easy-to-use, synthetically useful biocatalyst, and 8 out of the 16 alcohols obtained had enantiomeric purities of >98% ee.

Original languageEnglish
Pages (from-to)1075-1078
Number of pages4
JournalTetrahedron Asymmetry
Volume16
Issue number6
DOIs
Publication statusPublished - Mar 21 2005

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ASJC Scopus subject areas

  • Catalysis
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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