TY - JOUR
T1 - Astrapterocarpan isolated from Astragalus membranaceus inhibits proliferation of vascular smooth muscle cells
AU - Ohkawara, Susumu
AU - Okuma, Yasunobu
AU - Uehara, Takashi
AU - Yamagishi, Takashi
AU - Nomura, Yasuyuki
N1 - Funding Information:
This study was supported by the Hokkaido collaboration of regional entities for the advancement of technological excellence, Japan Science and Technology Corporation. This research was also supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports Science, and Technology, Japan (Y.O., Y.N.).
PY - 2005/11/21
Y1 - 2005/11/21
N2 - The inhibitory effects of astrapterocarpan, formononetin, and calycosin isolated from Astragalus membraneceus on platelet-derived growth factor (PDGF)-BB-induced proliferative response in rat vascular smooth muscle cells (A10 cells) were investigated. Astrapterocarpan significantly inhibited PDGF-BB-induced cell proliferation and DNA synthesis in a concentration- dependent manner. This inhibition was not attributed to toxicity. In contrast, formononetin and calycosin had no effect. We next examined the effect of astrapterocarpan on PDGF-BB signal transduction. Astrapterocarpan inhibited PDGF-BB-induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERIC1/2) mitogen-activated protein (MAP) kinase. However, this compound had no effect on phosphorylation of PDGF-β-receptor, Akt kinase and p38 MAP kinase. These results indicated that astrapterocarpan inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and that this effect may be mediated, at least in part, by inhibition of the ERK1/2 MAP kinase cascade.
AB - The inhibitory effects of astrapterocarpan, formononetin, and calycosin isolated from Astragalus membraneceus on platelet-derived growth factor (PDGF)-BB-induced proliferative response in rat vascular smooth muscle cells (A10 cells) were investigated. Astrapterocarpan significantly inhibited PDGF-BB-induced cell proliferation and DNA synthesis in a concentration- dependent manner. This inhibition was not attributed to toxicity. In contrast, formononetin and calycosin had no effect. We next examined the effect of astrapterocarpan on PDGF-BB signal transduction. Astrapterocarpan inhibited PDGF-BB-induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERIC1/2) mitogen-activated protein (MAP) kinase. However, this compound had no effect on phosphorylation of PDGF-β-receptor, Akt kinase and p38 MAP kinase. These results indicated that astrapterocarpan inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and that this effect may be mediated, at least in part, by inhibition of the ERK1/2 MAP kinase cascade.
KW - Astragalus membraneceus
KW - Astrapterocarpan
KW - Cardiovascular disease
KW - Platelet-derived growth factor
KW - Vascular smooth muscle cell
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U2 - 10.1016/j.ejphar.2005.08.063
DO - 10.1016/j.ejphar.2005.08.063
M3 - Article
C2 - 16297381
AN - SCOPUS:28044431625
SN - 0014-2999
VL - 525
SP - 41
EP - 47
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -
