Astrapterocarpan isolated from Astragalus membranaceus inhibits proliferation of vascular smooth muscle cells

Susumu Ohkawara, Yasunobu Okuma, Takashi Uehara, Takashi Yamagishi, Yasuyuki Nomura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The inhibitory effects of astrapterocarpan, formononetin, and calycosin isolated from Astragalus membraneceus on platelet-derived growth factor (PDGF)-BB-induced proliferative response in rat vascular smooth muscle cells (A10 cells) were investigated. Astrapterocarpan significantly inhibited PDGF-BB-induced cell proliferation and DNA synthesis in a concentration- dependent manner. This inhibition was not attributed to toxicity. In contrast, formononetin and calycosin had no effect. We next examined the effect of astrapterocarpan on PDGF-BB signal transduction. Astrapterocarpan inhibited PDGF-BB-induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERIC1/2) mitogen-activated protein (MAP) kinase. However, this compound had no effect on phosphorylation of PDGF-β-receptor, Akt kinase and p38 MAP kinase. These results indicated that astrapterocarpan inhibits PDGF-BB-induced vascular smooth muscle cell proliferation and that this effect may be mediated, at least in part, by inhibition of the ERK1/2 MAP kinase cascade.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalEuropean Journal of Pharmacology
Volume525
Issue number1-3
DOIs
Publication statusPublished - Nov 21 2005
Externally publishedYes

Keywords

  • Astragalus membraneceus
  • Astrapterocarpan
  • Cardiovascular disease
  • Platelet-derived growth factor
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Pharmacology

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