Association of spindale midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes

Junko Otsuki, Yasushi Nagai, Kazuyoshi Chiba

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Polo-like kinase 1 (Plk1) has been reported to localize to the spindle midzone during meiosis in mouse oocytes. However, it has not been reported in human oocytes. In this study, the interaction of the meiotic structures and chromosome segregation in mouse and human oocytes were studied by time-lapse differential interference contrast microscopy. Using immunocytochemical studies, the localization of polo-like kinase 1 and its association with microtubules were examined during the extrusion of first and second polar bodies. It was found that Plk1 was localized in the spindle midzone in human oocytes at anaphase I and telophage I. Also, three-dimensional confocal laser microscopy showed that the mciotic spindle midzone contained numerous dot-like particles that were stained by anti-Plk1 antibody. These particles were aligned in the plane of the meiotic midzone in mouse and human oocytes.

Original languageEnglish
Pages (from-to)522-528
Number of pages7
JournalReproductive BioMedicine Online
Volume18
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Keywords

  • Chromosome segregation
  • Meiotic spindle
  • Microfilaments
  • Midzone
  • Plk1
  • Polar body extrusion

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

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