Abstract
Polo-like kinase 1 (Plk1) has been reported to localize to the spindle midzone during meiosis in mouse oocytes. However, it has not been reported in human oocytes. In this study, the interaction of the meiotic structures and chromosome segregation in mouse and human oocytes were studied by time-lapse differential interference contrast microscopy. Using immunocytochemical studies, the localization of polo-like kinase 1 and its association with microtubules were examined during the extrusion of first and second polar bodies. It was found that Plk1 was localized in the spindle midzone in human oocytes at anaphase I and telophage I. Also, three-dimensional confocal laser microscopy showed that the mciotic spindle midzone contained numerous dot-like particles that were stained by anti-Plk1 antibody. These particles were aligned in the plane of the meiotic midzone in mouse and human oocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 522-528 |
| Number of pages | 7 |
| Journal | Reproductive BioMedicine Online |
| Volume | 18 |
| Issue number | 4 |
| Publication status | Published - Apr 2009 |
| Externally published | Yes |
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Keywords
- Chromosome segregation
- Meiotic spindle
- Microfilaments
- Midzone
- Plk1
- Polar body extrusion
ASJC Scopus subject areas
- Reproductive Medicine
- Developmental Biology
Cite this
Association of spindale midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes. / Otsuki, Junko; Nagai, Yasushi; Chiba, Kazuyoshi.
In: Reproductive BioMedicine Online, Vol. 18, No. 4, 04.2009, p. 522-528.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of spindale midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes
AU - Otsuki, Junko
AU - Nagai, Yasushi
AU - Chiba, Kazuyoshi
PY - 2009/4
Y1 - 2009/4
N2 - Polo-like kinase 1 (Plk1) has been reported to localize to the spindle midzone during meiosis in mouse oocytes. However, it has not been reported in human oocytes. In this study, the interaction of the meiotic structures and chromosome segregation in mouse and human oocytes were studied by time-lapse differential interference contrast microscopy. Using immunocytochemical studies, the localization of polo-like kinase 1 and its association with microtubules were examined during the extrusion of first and second polar bodies. It was found that Plk1 was localized in the spindle midzone in human oocytes at anaphase I and telophage I. Also, three-dimensional confocal laser microscopy showed that the mciotic spindle midzone contained numerous dot-like particles that were stained by anti-Plk1 antibody. These particles were aligned in the plane of the meiotic midzone in mouse and human oocytes.
AB - Polo-like kinase 1 (Plk1) has been reported to localize to the spindle midzone during meiosis in mouse oocytes. However, it has not been reported in human oocytes. In this study, the interaction of the meiotic structures and chromosome segregation in mouse and human oocytes were studied by time-lapse differential interference contrast microscopy. Using immunocytochemical studies, the localization of polo-like kinase 1 and its association with microtubules were examined during the extrusion of first and second polar bodies. It was found that Plk1 was localized in the spindle midzone in human oocytes at anaphase I and telophage I. Also, three-dimensional confocal laser microscopy showed that the mciotic spindle midzone contained numerous dot-like particles that were stained by anti-Plk1 antibody. These particles were aligned in the plane of the meiotic midzone in mouse and human oocytes.
KW - Chromosome segregation
KW - Meiotic spindle
KW - Microfilaments
KW - Midzone
KW - Plk1
KW - Polar body extrusion
UR - http://www.scopus.com/inward/record.url?scp=65249166974&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65249166974&partnerID=8YFLogxK
M3 - Article
VL - 18
SP - 522
EP - 528
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 4
ER -