Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis

Hiroyuki Nakamura; Fumiko Higashikawa; Kiyoshi Miyagawa; Yoshitaka Nobukuni; Tomohiko Endo; Toru Imai; Kotaro Ozasa; Yutaka Motohashi; Ichiyo Matsuzaki; Shinichiro Sasahara; Kotaro Hatta; Keiki Ogino; Akira Eboshida

doi:10.1159/000080222

Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis

Hiroyuki Nakamura, Fumiko Higashikawa, Kiyoshi Miyagawa, Yoshitaka Nobukuni, Tomohiko Endo, Toru Imai, Kotaro Ozasa, Yutaka Motohashi, Ichiyo Matsuzaki, Shinichiro Sasahara, Kotaro Hatta, Keiki Ogino, Akira Eboshida

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

Original language	English
Pages (from-to)	40-43
Number of pages	4
Journal	International archives of allergy and immunology
Volume	135
Issue number	1
DOIs	https://doi.org/10.1159/000080222
Publication status	Published - 2004

Keywords

Cedar pollinosis
Eosinophil peroxidase
Polymorphisms
Transmission disequilibrium test

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1159/000080222

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Nakamura, H., Higashikawa, F., Miyagawa, K., Nobukuni, Y., Endo, T., Imai, T., Ozasa, K., Motohashi, Y., Matsuzaki, I., Sasahara, S., Hatta, K., Ogino, K., & Eboshida, A. (2004). Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. International archives of allergy and immunology, 135(1), 40-43. https://doi.org/10.1159/000080222

Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. / Nakamura, Hiroyuki; Higashikawa, Fumiko; Miyagawa, Kiyoshi; Nobukuni, Yoshitaka; Endo, Tomohiko; Imai, Toru; Ozasa, Kotaro; Motohashi, Yutaka; Matsuzaki, Ichiyo; Sasahara, Shinichiro; Hatta, Kotaro; Ogino, Keiki; Eboshida, Akira.

In: International archives of allergy and immunology, Vol. 135, No. 1, 2004, p. 40-43.

Research output: Contribution to journal › Article › peer-review

Nakamura, H, Higashikawa, F, Miyagawa, K, Nobukuni, Y, Endo, T, Imai, T, Ozasa, K, Motohashi, Y, Matsuzaki, I, Sasahara, S, Hatta, K, Ogino, K & Eboshida, A 2004, 'Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis', International archives of allergy and immunology, vol. 135, no. 1, pp. 40-43. https://doi.org/10.1159/000080222

Nakamura, Hiroyuki ; Higashikawa, Fumiko ; Miyagawa, Kiyoshi ; Nobukuni, Yoshitaka ; Endo, Tomohiko ; Imai, Toru ; Ozasa, Kotaro ; Motohashi, Yutaka ; Matsuzaki, Ichiyo ; Sasahara, Shinichiro ; Hatta, Kotaro ; Ogino, Keiki ; Eboshida, Akira. / Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. In: International archives of allergy and immunology. 2004 ; Vol. 135, No. 1. pp. 40-43.

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abstract = "Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.",

keywords = "Cedar pollinosis, Eosinophil peroxidase, Polymorphisms, Transmission disequilibrium test",

author = "Hiroyuki Nakamura and Fumiko Higashikawa and Kiyoshi Miyagawa and Yoshitaka Nobukuni and Tomohiko Endo and Toru Imai and Kotaro Ozasa and Yutaka Motohashi and Ichiyo Matsuzaki and Shinichiro Sasahara and Kotaro Hatta and Keiki Ogino and Akira Eboshida",

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AU - Nakamura, Hiroyuki

AU - Higashikawa, Fumiko

AU - Miyagawa, Kiyoshi

AU - Nobukuni, Yoshitaka

AU - Endo, Tomohiko

AU - Imai, Toru

AU - Ozasa, Kotaro

AU - Motohashi, Yutaka

AU - Matsuzaki, Ichiyo

AU - Sasahara, Shinichiro

AU - Hatta, Kotaro

AU - Ogino, Keiki

AU - Eboshida, Akira

PY - 2004

Y1 - 2004

N2 - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

AB - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

KW - Cedar pollinosis

KW - Eosinophil peroxidase

KW - Polymorphisms

KW - Transmission disequilibrium test

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