TY - JOUR
T1 - Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis
AU - Nakamura, Hiroyuki
AU - Higashikawa, Fumiko
AU - Miyagawa, Kiyoshi
AU - Nobukuni, Yoshitaka
AU - Endo, Tomohiko
AU - Imai, Toru
AU - Ozasa, Kotaro
AU - Motohashi, Yutaka
AU - Matsuzaki, Ichiyo
AU - Sasahara, Shinichiro
AU - Hatta, Kotaro
AU - Ogino, Keiki
AU - Eboshida, Akira
PY - 2004
Y1 - 2004
N2 - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
AB - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
KW - Cedar pollinosis
KW - Eosinophil peroxidase
KW - Polymorphisms
KW - Transmission disequilibrium test
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U2 - 10.1159/000080222
DO - 10.1159/000080222
M3 - Article
C2 - 15316147
AN - SCOPUS:4644355932
VL - 135
SP - 40
EP - 43
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 1
ER -