Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis

Hiroyuki Nakamura, Fumiko Higashikawa, Kiyoshi Miyagawa, Yoshitaka Nobukuni, Tomohiko Endo, Toru Imai, Kotaro Ozasa, Yutaka Motohashi, Ichiyo Matsuzaki, Shinichiro Sasahara, Kotaro Hatta, Keiki Ogino, Akira Eboshida

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

Original languageEnglish
Pages (from-to)40-43
Number of pages4
JournalInternational Archives of Allergy and Immunology
Volume135
Issue number1
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Eosinophil Peroxidase
Cryptomeria
Seasonal Allergic Rhinitis
Single Nucleotide Polymorphism
Exons
Genes
Restriction Fragment Length Polymorphisms
Japan
Parents
Polymerase Chain Reaction
Population

Keywords

  • Cedar pollinosis
  • Eosinophil peroxidase
  • Polymorphisms
  • Transmission disequilibrium test

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. / Nakamura, Hiroyuki; Higashikawa, Fumiko; Miyagawa, Kiyoshi; Nobukuni, Yoshitaka; Endo, Tomohiko; Imai, Toru; Ozasa, Kotaro; Motohashi, Yutaka; Matsuzaki, Ichiyo; Sasahara, Shinichiro; Hatta, Kotaro; Ogino, Keiki; Eboshida, Akira.

In: International Archives of Allergy and Immunology, Vol. 135, No. 1, 2004, p. 40-43.

Research output: Contribution to journalArticle

Nakamura, H, Higashikawa, F, Miyagawa, K, Nobukuni, Y, Endo, T, Imai, T, Ozasa, K, Motohashi, Y, Matsuzaki, I, Sasahara, S, Hatta, K, Ogino, K & Eboshida, A 2004, 'Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis', International Archives of Allergy and Immunology, vol. 135, no. 1, pp. 40-43. https://doi.org/10.1159/000080222
Nakamura, Hiroyuki ; Higashikawa, Fumiko ; Miyagawa, Kiyoshi ; Nobukuni, Yoshitaka ; Endo, Tomohiko ; Imai, Toru ; Ozasa, Kotaro ; Motohashi, Yutaka ; Matsuzaki, Ichiyo ; Sasahara, Shinichiro ; Hatta, Kotaro ; Ogino, Keiki ; Eboshida, Akira. / Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. In: International Archives of Allergy and Immunology. 2004 ; Vol. 135, No. 1. pp. 40-43.
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abstract = "Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.",
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AU - Miyagawa, Kiyoshi

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AU - Endo, Tomohiko

AU - Imai, Toru

AU - Ozasa, Kotaro

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AU - Matsuzaki, Ichiyo

AU - Sasahara, Shinichiro

AU - Hatta, Kotaro

AU - Ogino, Keiki

AU - Eboshida, Akira

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N2 - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

AB - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.

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KW - Transmission disequilibrium test

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