Abstract
Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
| Original language | English |
|---|---|
| Pages (from-to) | 40-43 |
| Number of pages | 4 |
| Journal | International Archives of Allergy and Immunology |
| Volume | 135 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2004 |
| Externally published | Yes |
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Keywords
- Cedar pollinosis
- Eosinophil peroxidase
- Polymorphisms
- Transmission disequilibrium test
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis. / Nakamura, Hiroyuki; Higashikawa, Fumiko; Miyagawa, Kiyoshi; Nobukuni, Yoshitaka; Endo, Tomohiko; Imai, Toru; Ozasa, Kotaro; Motohashi, Yutaka; Matsuzaki, Ichiyo; Sasahara, Shinichiro; Hatta, Kotaro; Ogino, Keiki; Eboshida, Akira.
In: International Archives of Allergy and Immunology, Vol. 135, No. 1, 2004, p. 40-43.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis
AU - Nakamura, Hiroyuki
AU - Higashikawa, Fumiko
AU - Miyagawa, Kiyoshi
AU - Nobukuni, Yoshitaka
AU - Endo, Tomohiko
AU - Imai, Toru
AU - Ozasa, Kotaro
AU - Motohashi, Yutaka
AU - Matsuzaki, Ichiyo
AU - Sasahara, Shinichiro
AU - Hatta, Kotaro
AU - Ogino, Keiki
AU - Eboshida, Akira
PY - 2004
Y1 - 2004
N2 - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
AB - Background: Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population. Methods: We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method. Results: We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children. Conclusions: Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
KW - Cedar pollinosis
KW - Eosinophil peroxidase
KW - Polymorphisms
KW - Transmission disequilibrium test
UR - http://www.scopus.com/inward/record.url?scp=4644355932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4644355932&partnerID=8YFLogxK
U2 - 10.1159/000080222
DO - 10.1159/000080222
M3 - Article
C2 - 15316147
AN - SCOPUS:4644355932
VL - 135
SP - 40
EP - 43
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 1
ER -