Association of Rab3A with synaptic vesicles at late stages of the secretory pathway

Michela Matteoli, Kohji Takei, Richard Cameron, Paul Hurlbut, Patricia A. Johnston, Thomas C. Südhof, Reinhard Jahn, Pietro De Camilli

Research output: Contribution to journalArticle

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Abstract

Rab3A is a small GTP-binding protein highly concentrated on synaptic vesicles. Like other small GTP-binding proteins it is thought to cycle between a soluble and a membrane-associated state. To determine at which stage of the life cycle of synaptic vesicles rab3A is associated with their membranes, the localization of the protein in neurons and neuroendocrine cells at different developmental and functional stages was investigated. In all cases, rab3A was colocalized with synaptic vesicle markers at the cell periphery, but was absent from the Golgi area, suggesting that rab3A associates with vesicles distally to the Golgi complex and dissociates from vesicle membranes before they recycle to this region. Immunofluorescence experiments carried out on frog motor end plates demonstrated that massive exocytosis of synaptic vesicles is accompanied by a translocation of rab3A to the cell surface. The selective localization of rab3A on synaptic vesicles at stages preceding their fusion with the plasmalemma suggests that the protein is part of a regulatory machinery that is assembled onto the vesicles in preparation for exocytosis.

Original languageEnglish
Pages (from-to)625-633
Number of pages9
JournalJournal of Cell Biology
Volume115
Issue number3
Publication statusPublished - Nov 1991
Externally publishedYes

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Synaptic Vesicles
Secretory Pathway
Exocytosis
GTP-Binding Proteins
Motor Endplate
Neuroendocrine Cells
Membranes
Golgi Apparatus
Life Cycle Stages
Anura
Fluorescent Antibody Technique
Membrane Proteins
Neurons
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Matteoli, M., Takei, K., Cameron, R., Hurlbut, P., Johnston, P. A., Südhof, T. C., ... De Camilli, P. (1991). Association of Rab3A with synaptic vesicles at late stages of the secretory pathway. Journal of Cell Biology, 115(3), 625-633.

Association of Rab3A with synaptic vesicles at late stages of the secretory pathway. / Matteoli, Michela; Takei, Kohji; Cameron, Richard; Hurlbut, Paul; Johnston, Patricia A.; Südhof, Thomas C.; Jahn, Reinhard; De Camilli, Pietro.

In: Journal of Cell Biology, Vol. 115, No. 3, 11.1991, p. 625-633.

Research output: Contribution to journalArticle

Matteoli, M, Takei, K, Cameron, R, Hurlbut, P, Johnston, PA, Südhof, TC, Jahn, R & De Camilli, P 1991, 'Association of Rab3A with synaptic vesicles at late stages of the secretory pathway', Journal of Cell Biology, vol. 115, no. 3, pp. 625-633.
Matteoli M, Takei K, Cameron R, Hurlbut P, Johnston PA, Südhof TC et al. Association of Rab3A with synaptic vesicles at late stages of the secretory pathway. Journal of Cell Biology. 1991 Nov;115(3):625-633.
Matteoli, Michela ; Takei, Kohji ; Cameron, Richard ; Hurlbut, Paul ; Johnston, Patricia A. ; Südhof, Thomas C. ; Jahn, Reinhard ; De Camilli, Pietro. / Association of Rab3A with synaptic vesicles at late stages of the secretory pathway. In: Journal of Cell Biology. 1991 ; Vol. 115, No. 3. pp. 625-633.
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