Association of nuclear membrane protein lamin B1 with necrosis and apoptosis in cell death induced by 5-fluoro-2′-deoxyuridine

Akira Sato, Akiko Hiramoto, Akito Satake, Eriko Miyazaki, Tomoharu Naito, Yusuke Wataya, Hye Sook Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We report that anticancer 5-fluoro-2′-deoxyuridine (FUdR) shows cytotoxicity against mouse cancer cell line FM3A, using a progeny clone F28-7 and its variant F28-7-A. In this process, the cell-death morphology is different between F28-7 and F28-7-A cells, that is, necrosis in F28-7 but apoptosis in F28-7-A cells. In the proteomic analysis of these cells before their exposure to FUdR, the nuclear inner-membrane protein lamin B1 is up-regulated in F28-7 but not in F28-7-A, suggesting that lamin B1 may possess a function to regulate the morphology of cell-death. A knockdown of lamin B1 expression in F28-7 cells was performed by use of the small interfering RNA technique, resulting in a decrease of the lamin B1-expression level down to the level in F28-7-A. Remarkably, the FUdR-induced death morphology of this knocked-down F28-7 was apoptosis, definitely different from the necrosis that occurs in the FUdR-treated original F28-7. Thus, the swelling feature for the necrosis was no longer observable, and instead cell shrinkage typical of apoptosis took place in almost all the cells examined. This finding suggests a new role for lamin B1 as a regulator in cell death.

Original languageEnglish
Pages (from-to)433-438
Number of pages6
JournalNucleosides, Nucleotides and Nucleic Acids
Volume27
Issue number5
DOIs
Publication statusPublished - May 1 2008

Keywords

  • 5-Fluoro-2′-deoxyuridine
  • Apoptosis
  • Lamin B1
  • Necrosis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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