Association of N-ethylmaleimide-sensitive factor with synaptic vesicles

Rong Mao Hong, Hiroyuki Mori, Toshio Fukui, Yoshinori Moriyama, Masamitsu Futai, Akitsugu Yamamoto, Yutaka Tashiro, Mitsuo Tagaya

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

N-Ethylmaleimide-sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi-pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti-NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca2+ influx.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalFEBS Letters
Volume350
Issue number2-3
DOIs
Publication statusPublished - Aug 22 1994
Externally publishedYes

Keywords

  • Neurotransmitter
  • Synaptic vesicle
  • Vesicular transport

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Hong, R. M., Mori, H., Fukui, T., Moriyama, Y., Futai, M., Yamamoto, A., Tashiro, Y., & Tagaya, M. (1994). Association of N-ethylmaleimide-sensitive factor with synaptic vesicles. FEBS Letters, 350(2-3), 253-257. https://doi.org/10.1016/0014-5793(94)00778-0