TY - JOUR
T1 - Association of Lactobacillus buchneri with aerobic stability of total mixed ration containing wet brewers grains preserved as a silage
AU - Wang, Fujin
AU - Nishino, Naoki
N1 - Funding Information:
A part of this study was financially supported by a Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science (No. 19580311), and by the Sasakawa Scientific Research Grant from the Japan Science Society (No. 18-251).
PY - 2009/3/16
Y1 - 2009/3/16
N2 - Laboratory-scale plastic silos were prepared to examine bacterial factors associated with the aerobic stability of total mixed rations (TMR) preserved as a silage after mixing. In experiment 1, TMR silages containing wet brewers grains (BG), hay (lucerne or sudangrass), maize grain, wheat bran, beet pulp and liquid molasses, were ensiled for 14 and 56 days, and bacterial communities were examined by denaturing gradient gel electrophoresis (DGGE). The silages resisted aerobic deterioration for as long as 2 weeks, except that heating occurred after 5.5 days in 14-day silage prepared with sudangrass hay. The DGGE analyses indicated that, although not found in the materials, Lactobacillus buchneri and L. brevis were detectable in long-stored TMR silage. L. delbrueckii and L. fermentum were found in the materials, and appeared to survive during fermentation and after exposure to air. There were few changes in the DGGE profiles before and after heating in deteriorated silage containing sudangrass hay. In experiment 2, TMR silage with ingredients similar to those in experiment 1 was prepared, and culturable lactic acid bacteria were isolated from the plates of de Man-Rogosa-Sharpe agar. L. delbrueckii and L. fermentum were obtained from the materials but not from TMR silage. L. buchneri and L. brevis were not found in the materials, but more than one-third of the isolates were L. buchneri and L. brevis in 14- and 56-day silages, respectively. In experiment 3, whole crop maize was inoculated with L. buchneri, L. brevis, L. delbrueckii and L. fermentum isolates, and fermentation and aerobic stability were determined. No changes occurred from L. brevis, L. delbrueckii and L. fermentum inoculation, while aerobic spoilage was suppressed by L. buchneri with increases in pH and acetic acid content. Results indicate that, whilst not found in BG or the TMR mixture, L. buchneri can become detectable during fermentation, and this bacterium helps establish aerobic stability of BG-containing TMR silage.
AB - Laboratory-scale plastic silos were prepared to examine bacterial factors associated with the aerobic stability of total mixed rations (TMR) preserved as a silage after mixing. In experiment 1, TMR silages containing wet brewers grains (BG), hay (lucerne or sudangrass), maize grain, wheat bran, beet pulp and liquid molasses, were ensiled for 14 and 56 days, and bacterial communities were examined by denaturing gradient gel electrophoresis (DGGE). The silages resisted aerobic deterioration for as long as 2 weeks, except that heating occurred after 5.5 days in 14-day silage prepared with sudangrass hay. The DGGE analyses indicated that, although not found in the materials, Lactobacillus buchneri and L. brevis were detectable in long-stored TMR silage. L. delbrueckii and L. fermentum were found in the materials, and appeared to survive during fermentation and after exposure to air. There were few changes in the DGGE profiles before and after heating in deteriorated silage containing sudangrass hay. In experiment 2, TMR silage with ingredients similar to those in experiment 1 was prepared, and culturable lactic acid bacteria were isolated from the plates of de Man-Rogosa-Sharpe agar. L. delbrueckii and L. fermentum were obtained from the materials but not from TMR silage. L. buchneri and L. brevis were not found in the materials, but more than one-third of the isolates were L. buchneri and L. brevis in 14- and 56-day silages, respectively. In experiment 3, whole crop maize was inoculated with L. buchneri, L. brevis, L. delbrueckii and L. fermentum isolates, and fermentation and aerobic stability were determined. No changes occurred from L. brevis, L. delbrueckii and L. fermentum inoculation, while aerobic spoilage was suppressed by L. buchneri with increases in pH and acetic acid content. Results indicate that, whilst not found in BG or the TMR mixture, L. buchneri can become detectable during fermentation, and this bacterium helps establish aerobic stability of BG-containing TMR silage.
KW - Aerobic stability
KW - Denaturing gradient gel electrophoresis
KW - Lactic acid bacteria
KW - Silage
KW - Total mixed ration
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U2 - 10.1016/j.anifeedsci.2008.06.012
DO - 10.1016/j.anifeedsci.2008.06.012
M3 - Article
AN - SCOPUS:60849083524
VL - 149
SP - 265
EP - 274
JO - Animal Feed Science and Technology
JF - Animal Feed Science and Technology
SN - 0377-8401
IS - 3-4
ER -