Abstract
Fifty Japanese patients with insulin-dependent diabetes mellitus (IDDM) and 94 normal subjects were genotyped for BglII restriction-fragment-length polymorphism (RFLP) of the T-lymphocyte-receptor β-chain (TLRβ)-region gene and analyzed in relation to HLA-DR phenotypes. The antigen frequencies of DR4 and DR9 in the IDDM population were significantly higher than those in the normal population, with relative risks of 1.87 (P <.02) and 2.42 (P <.01), respectively. Hybridization of digested DNA with the TLRβ probe revealed two alleles of 9.3 and 8.6 kilobases (kb). The allele frequency of 8.6 kb in patients with IDDM (79%) was significantly (P <.05) higher than that in normal subjects (64%). When TLRβ-region RFLP in IDDM was further analyzed with respect to the HLA-DR phenotypes, the frequency of 8.6 kb was significantly increased in patients with DR4 but not DR9 (DR4/X) and those with DR9 but not DR4 (DR9/X) compared with the frequency found in normal subjects (P <.05); the relative risks of 8.6 kb in DR4/X and DR9/X were 2.77 and 4.98, respectively. Although the frequencies of HLA-DR phenotypes and of TLRβ-region RFLP in IDDM and normal subjects were apparently different from those reported for Caucasians, this population-association study indicates that in the Japanese, genes conferring susceptibility to IDDM exist near or at the HLA-DR and the TLRβ loci, as has been demonstrated in Caucasians.
| Original language | English |
|---|---|
| Pages (from-to) | 1633-1636 |
| Number of pages | 4 |
| Journal | Diabetes |
| Volume | 37 |
| Issue number | 12 |
| Publication status | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Internal Medicine
Cite this
Association of HLA-DR phenotypes and T-lymphocyte-receptor β-chain-region RFLP with IDDM in Japanese. / Ito, M.; Tanimoto, M.; Kamura, H.; Yoneda, M.; Morishima, Y.; Takatsuki, K.; Itatsu, T.; Saito, H.
In: Diabetes, Vol. 37, No. 12, 1988, p. 1633-1636.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of HLA-DR phenotypes and T-lymphocyte-receptor β-chain-region RFLP with IDDM in Japanese
AU - Ito, M.
AU - Tanimoto, M.
AU - Kamura, H.
AU - Yoneda, M.
AU - Morishima, Y.
AU - Takatsuki, K.
AU - Itatsu, T.
AU - Saito, H.
PY - 1988
Y1 - 1988
N2 - Fifty Japanese patients with insulin-dependent diabetes mellitus (IDDM) and 94 normal subjects were genotyped for BglII restriction-fragment-length polymorphism (RFLP) of the T-lymphocyte-receptor β-chain (TLRβ)-region gene and analyzed in relation to HLA-DR phenotypes. The antigen frequencies of DR4 and DR9 in the IDDM population were significantly higher than those in the normal population, with relative risks of 1.87 (P <.02) and 2.42 (P <.01), respectively. Hybridization of digested DNA with the TLRβ probe revealed two alleles of 9.3 and 8.6 kilobases (kb). The allele frequency of 8.6 kb in patients with IDDM (79%) was significantly (P <.05) higher than that in normal subjects (64%). When TLRβ-region RFLP in IDDM was further analyzed with respect to the HLA-DR phenotypes, the frequency of 8.6 kb was significantly increased in patients with DR4 but not DR9 (DR4/X) and those with DR9 but not DR4 (DR9/X) compared with the frequency found in normal subjects (P <.05); the relative risks of 8.6 kb in DR4/X and DR9/X were 2.77 and 4.98, respectively. Although the frequencies of HLA-DR phenotypes and of TLRβ-region RFLP in IDDM and normal subjects were apparently different from those reported for Caucasians, this population-association study indicates that in the Japanese, genes conferring susceptibility to IDDM exist near or at the HLA-DR and the TLRβ loci, as has been demonstrated in Caucasians.
AB - Fifty Japanese patients with insulin-dependent diabetes mellitus (IDDM) and 94 normal subjects were genotyped for BglII restriction-fragment-length polymorphism (RFLP) of the T-lymphocyte-receptor β-chain (TLRβ)-region gene and analyzed in relation to HLA-DR phenotypes. The antigen frequencies of DR4 and DR9 in the IDDM population were significantly higher than those in the normal population, with relative risks of 1.87 (P <.02) and 2.42 (P <.01), respectively. Hybridization of digested DNA with the TLRβ probe revealed two alleles of 9.3 and 8.6 kilobases (kb). The allele frequency of 8.6 kb in patients with IDDM (79%) was significantly (P <.05) higher than that in normal subjects (64%). When TLRβ-region RFLP in IDDM was further analyzed with respect to the HLA-DR phenotypes, the frequency of 8.6 kb was significantly increased in patients with DR4 but not DR9 (DR4/X) and those with DR9 but not DR4 (DR9/X) compared with the frequency found in normal subjects (P <.05); the relative risks of 8.6 kb in DR4/X and DR9/X were 2.77 and 4.98, respectively. Although the frequencies of HLA-DR phenotypes and of TLRβ-region RFLP in IDDM and normal subjects were apparently different from those reported for Caucasians, this population-association study indicates that in the Japanese, genes conferring susceptibility to IDDM exist near or at the HLA-DR and the TLRβ loci, as has been demonstrated in Caucasians.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024203201&partnerID=8YFLogxK
M3 - Article
C2 - 2903835
AN - SCOPUS:0024203201
VL - 37
SP - 1633
EP - 1636
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 12
ER -