Association of CYP2A6 gene deletion with cigarette smoking status in Japanese adults

Masahiko Ando, Nobuyuki Hamajima, Noritaka Ariyoshi, Tetsuya Kamataki, Keitaro Matsuo, Yoshiyuki Ohno

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

BACKGROUND: Genetic variation of CYP2A6 is shown to alter nicotine metabolism. This study was developed to investigate the genetic influence of the whole deletion-allele of CYP2A6 on active and passive smoking behavior. METHODS: Two hundred and forty Japanese adults, who visited Aichi Cancer Center as outpatients, were genotyped for the wild-type (CYP2A6*1A, CYP2A6*1B) and the whole deletion-type (CYP2A6*4C) polymorphism of CYP2A6. Information about active and passive smoking status was obtained by a self-administered questionnaire. Genetic influence of CYP2A6 polymorphism on smoking behavior was evaluated using the Mantel extension test. RESULTS: The frequency of the deletion allele was 18%. All 8 subjects carrying two deletion alleles had no smoking habit, and the homozygous deletion genotype showed a tendency to correlate with active smoking status after adjustment for sex and age (p=0.054). However, the proportion of never smokers among heterozygous subjects was almost the same as among subjects carrying no deletion allele (54% and 58%, respectively). Furthermore, CYP2A6 genotypes were correlated neither with the number of cigarettes smoked per day nor with the age at starting smoking (p=0.364 and 0.880, respectively). Among never smokers, CYP2A6 genotypes were not correlated with exposure to passive smoking at home or in the workplace (p=0.623 and 0.484, respectively). CONCLUSION: Despite the possible protection against active smoking behavior in subjects homozygous for the deletion allele, the CYP2A6 polymorphism has only a limited impact on public health because no protective effect was found in heterozygous subjects.

Original languageEnglish
Pages (from-to)176-181
Number of pages6
JournalJournal of Epidemiology
Volume13
Issue number3
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Gene Deletion
Smoking
Tobacco Smoke Pollution
Alleles
Genotype
Nicotine
Gene Frequency
Tobacco Products
Workplace
Habits
Outpatients
Public Health
Neoplasms

Keywords

  • Cytochrome P450 2A6
  • Genetic polymorphism
  • Smoking behavior

ASJC Scopus subject areas

  • Epidemiology

Cite this

Ando, M., Hamajima, N., Ariyoshi, N., Kamataki, T., Matsuo, K., & Ohno, Y. (2003). Association of CYP2A6 gene deletion with cigarette smoking status in Japanese adults. Journal of Epidemiology, 13(3), 176-181.

Association of CYP2A6 gene deletion with cigarette smoking status in Japanese adults. / Ando, Masahiko; Hamajima, Nobuyuki; Ariyoshi, Noritaka; Kamataki, Tetsuya; Matsuo, Keitaro; Ohno, Yoshiyuki.

In: Journal of Epidemiology, Vol. 13, No. 3, 2003, p. 176-181.

Research output: Contribution to journalArticle

Ando, M, Hamajima, N, Ariyoshi, N, Kamataki, T, Matsuo, K & Ohno, Y 2003, 'Association of CYP2A6 gene deletion with cigarette smoking status in Japanese adults', Journal of Epidemiology, vol. 13, no. 3, pp. 176-181.
Ando, Masahiko ; Hamajima, Nobuyuki ; Ariyoshi, Noritaka ; Kamataki, Tetsuya ; Matsuo, Keitaro ; Ohno, Yoshiyuki. / Association of CYP2A6 gene deletion with cigarette smoking status in Japanese adults. In: Journal of Epidemiology. 2003 ; Vol. 13, No. 3. pp. 176-181.
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abstract = "BACKGROUND: Genetic variation of CYP2A6 is shown to alter nicotine metabolism. This study was developed to investigate the genetic influence of the whole deletion-allele of CYP2A6 on active and passive smoking behavior. METHODS: Two hundred and forty Japanese adults, who visited Aichi Cancer Center as outpatients, were genotyped for the wild-type (CYP2A6*1A, CYP2A6*1B) and the whole deletion-type (CYP2A6*4C) polymorphism of CYP2A6. Information about active and passive smoking status was obtained by a self-administered questionnaire. Genetic influence of CYP2A6 polymorphism on smoking behavior was evaluated using the Mantel extension test. RESULTS: The frequency of the deletion allele was 18{\%}. All 8 subjects carrying two deletion alleles had no smoking habit, and the homozygous deletion genotype showed a tendency to correlate with active smoking status after adjustment for sex and age (p=0.054). However, the proportion of never smokers among heterozygous subjects was almost the same as among subjects carrying no deletion allele (54{\%} and 58{\%}, respectively). Furthermore, CYP2A6 genotypes were correlated neither with the number of cigarettes smoked per day nor with the age at starting smoking (p=0.364 and 0.880, respectively). Among never smokers, CYP2A6 genotypes were not correlated with exposure to passive smoking at home or in the workplace (p=0.623 and 0.484, respectively). CONCLUSION: Despite the possible protection against active smoking behavior in subjects homozygous for the deletion allele, the CYP2A6 polymorphism has only a limited impact on public health because no protective effect was found in heterozygous subjects.",
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AU - Ando, Masahiko

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AU - Matsuo, Keitaro

AU - Ohno, Yoshiyuki

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N2 - BACKGROUND: Genetic variation of CYP2A6 is shown to alter nicotine metabolism. This study was developed to investigate the genetic influence of the whole deletion-allele of CYP2A6 on active and passive smoking behavior. METHODS: Two hundred and forty Japanese adults, who visited Aichi Cancer Center as outpatients, were genotyped for the wild-type (CYP2A6*1A, CYP2A6*1B) and the whole deletion-type (CYP2A6*4C) polymorphism of CYP2A6. Information about active and passive smoking status was obtained by a self-administered questionnaire. Genetic influence of CYP2A6 polymorphism on smoking behavior was evaluated using the Mantel extension test. RESULTS: The frequency of the deletion allele was 18%. All 8 subjects carrying two deletion alleles had no smoking habit, and the homozygous deletion genotype showed a tendency to correlate with active smoking status after adjustment for sex and age (p=0.054). However, the proportion of never smokers among heterozygous subjects was almost the same as among subjects carrying no deletion allele (54% and 58%, respectively). Furthermore, CYP2A6 genotypes were correlated neither with the number of cigarettes smoked per day nor with the age at starting smoking (p=0.364 and 0.880, respectively). Among never smokers, CYP2A6 genotypes were not correlated with exposure to passive smoking at home or in the workplace (p=0.623 and 0.484, respectively). CONCLUSION: Despite the possible protection against active smoking behavior in subjects homozygous for the deletion allele, the CYP2A6 polymorphism has only a limited impact on public health because no protective effect was found in heterozygous subjects.

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