Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant

Tatsuya Atsumi, Masahiro Ieko, Maria L. Bertolaccini, Kenji Ichikawa, Akito Tsutsumi, Eiji Matsuura, Takao Koike

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95% confidence interval [95% CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1% for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95% CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.

Original languageEnglish
Pages (from-to)1982-1993
Number of pages12
JournalArthritis and Rheumatism
Volume43
Issue number9
DOIs
Publication statusPublished - 2000

Fingerprint

Lupus Coagulation Inhibitor
Antiphospholipid Syndrome
Phosphatidylserines
Prothrombin
Autoantibodies
Antibodies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Autoimmune Diseases
Immunoglobulin M
Glycoproteins
Odds Ratio
Confidence Intervals
Anticardiolipin Antibodies
Prothrombin Time
Immunoglobulin A
Antigens

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant. / Atsumi, Tatsuya; Ieko, Masahiro; Bertolaccini, Maria L.; Ichikawa, Kenji; Tsutsumi, Akito; Matsuura, Eiji; Koike, Takao.

In: Arthritis and Rheumatism, Vol. 43, No. 9, 2000, p. 1982-1993.

Research output: Contribution to journalArticle

Atsumi, Tatsuya ; Ieko, Masahiro ; Bertolaccini, Maria L. ; Ichikawa, Kenji ; Tsutsumi, Akito ; Matsuura, Eiji ; Koike, Takao. / Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant. In: Arthritis and Rheumatism. 2000 ; Vol. 43, No. 9. pp. 1982-1993.
@article{051c1d6f005646c782c69607fb9577af,
title = "Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant",
abstract = "Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95{\%} confidence interval [95{\%} CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1{\%} for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95{\%} CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.",
author = "Tatsuya Atsumi and Masahiro Ieko and Bertolaccini, {Maria L.} and Kenji Ichikawa and Akito Tsutsumi and Eiji Matsuura and Takao Koike",
year = "2000",
doi = "10.1002/1529-0131(200009)43:9<1982::AID-ANR9>3.0.CO;2-2",
language = "English",
volume = "43",
pages = "1982--1993",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "9",

}

TY - JOUR

T1 - Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant

AU - Atsumi, Tatsuya

AU - Ieko, Masahiro

AU - Bertolaccini, Maria L.

AU - Ichikawa, Kenji

AU - Tsutsumi, Akito

AU - Matsuura, Eiji

AU - Koike, Takao

PY - 2000

Y1 - 2000

N2 - Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95% confidence interval [95% CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1% for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95% CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.

AB - Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95% confidence interval [95% CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1% for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95% CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.

UR - http://www.scopus.com/inward/record.url?scp=0033711669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033711669&partnerID=8YFLogxK

U2 - 10.1002/1529-0131(200009)43:9<1982::AID-ANR9>3.0.CO;2-2

DO - 10.1002/1529-0131(200009)43:9<1982::AID-ANR9>3.0.CO;2-2

M3 - Article

C2 - 11014348

AN - SCOPUS:0033711669

VL - 43

SP - 1982

EP - 1993

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 9

ER -