Abstract
Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95% confidence interval [95% CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1% for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95% CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 1982-1993 |
| Number of pages | 12 |
| Journal | Arthritis and Rheumatism |
| Volume | 43 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2000 |
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ASJC Scopus subject areas
- Immunology
- Rheumatology
Cite this
Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant. / Atsumi, Tatsuya; Ieko, Masahiro; Bertolaccini, Maria L.; Ichikawa, Kenji; Tsutsumi, Akito; Matsuura, Eiji; Koike, Takao.
In: Arthritis and Rheumatism, Vol. 43, No. 9, 2000, p. 1982-1993.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of autoantibodies against the phosphatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant
AU - Atsumi, Tatsuya
AU - Ieko, Masahiro
AU - Bertolaccini, Maria L.
AU - Ichikawa, Kenji
AU - Tsutsumi, Akito
AU - Matsuura, Eiji
AU - Koike, Takao
PY - 2000
Y1 - 2000
N2 - Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95% confidence interval [95% CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1% for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95% CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.
AB - Objective. To clarity the association of autoantibodies against prothrombin with the clinical manifestations of the antiphospholipid syndrome (APS) and with the presence of lupus anticoagulant (LAC). Methods. We examined 265 patients who visited our autoimmune disease clinic. IgG and IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserineprothrombin complex (aPS/PT) antibodies or as antibodies against prothrombin coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, and IgA anticardiolipin (aCL) antibodies and their β2-glycoprotein I (β2GPI) dependency were also evaluated by ELISA. LAC was tested by 3 different methods. Results. The presence of aPS/PT, but not of aPT, significantly correlated with the clinical manifestations of APS (odds ratio [OR] 4.39, 95% confidence interval [95% CI] 2.06-9.38), and aPS/PT antibodies were as specific as β2GPI-dependent aCL for APS (93.1% for both). IgG aPS/PT strongly correlated with the presence of LAC as detected using the dilute Russell viper venom time test (OR 38.2, 95% CI 13.4-109.1). Conclusion. Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.
UR - http://www.scopus.com/inward/record.url?scp=0033711669&partnerID=8YFLogxK
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U2 - 10.1002/1529-0131(200009)43:9<1982::AID-ANR9>3.0.CO;2-2
DO - 10.1002/1529-0131(200009)43:9<1982::AID-ANR9>3.0.CO;2-2
M3 - Article
C2 - 11014348
AN - SCOPUS:0033711669
VL - 43
SP - 1982
EP - 1993
JO - Arthritis and Rheumatology
JF - Arthritis and Rheumatology
SN - 2326-5191
IS - 9
ER -