Association between Ion Fluxes and Defense Responses in Pea and Cowpea Tissues

Masashi Amano, Kazuhiro Toyoda, Yuki Ichinose, Tetsuji Yamada, Tomonori Shiraishi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The glycopeptide elicitor from a pea pathogen, Mycosphaerella pinodes, induced rapid alkalinization and increases in levels of Na+ and K+ ions in the extracellular solution upon contact with pea and cowpea tissues. The presence of monensin, nigericin, lidocaine, quinidine or phenytoin together with the elicitor markedly inhibited these changes, whereas the presence of valinomycin, gramicidin D, tetraethylammonium, CsCl and aminopyridine did not. The production of phytoalexins in pea and cowpea tissues was also strongly inhibited by the simultaneous presence of the former reagents but not of the latter reagents. Inhibitory effects on the production of phytoalexins were diminished when monensin, nigericin or a Na+-channel blocker was applied 3 h after the start of treatment with elicitor. Furthermore, orthovanadate and neomycin, which suppress defense responses in both tissues, also inhibited the above mentioned changes. By contrast, the species-specific suppressor from M. pinodes inhibited the elicitor-induced release of Na+ and K+ ions from pea tissues, but, conversely, by itself it elicited either the defense response or the release of Na+ and K+ ions from cowpea tissues. The results indicate that these ion-related changes, in particular the efflux of Na+ and K+ ions, might be closely associated with the signal transduction system for defense responses at the tissue level.

Original languageEnglish
Pages (from-to)698-706
Number of pages9
JournalPlant and Cell Physiology
Volume38
Issue number6
Publication statusPublished - Jun 1997

Fingerprint

Peas
cowpeas
peas
Ions
ions
Mycosphaerella pinodes
nigericin
Nigericin
Monensin
monensin
phytoalexins
gramicidin
phenytoin
Aminopyridines
quinidine
valinomycin
glycopeptides
Valinomycin
Gramicidin
alkalinization

Keywords

  • Defense response
  • Elicitor
  • Ion flux
  • Pisum sativum L.
  • Suppressor
  • Vigna sinensis endl

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Physiology

Cite this

Association between Ion Fluxes and Defense Responses in Pea and Cowpea Tissues. / Amano, Masashi; Toyoda, Kazuhiro; Ichinose, Yuki; Yamada, Tetsuji; Shiraishi, Tomonori.

In: Plant and Cell Physiology, Vol. 38, No. 6, 06.1997, p. 698-706.

Research output: Contribution to journalArticle

Amano, Masashi ; Toyoda, Kazuhiro ; Ichinose, Yuki ; Yamada, Tetsuji ; Shiraishi, Tomonori. / Association between Ion Fluxes and Defense Responses in Pea and Cowpea Tissues. In: Plant and Cell Physiology. 1997 ; Vol. 38, No. 6. pp. 698-706.
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